Publication: Comparison of physiological, cytopathogenic and immunological properties between two environmental isolates of Acanthamoeba spp.
Issued Date
2005-12-01
Resource Type
ISSN
01251562
Other identifier(s)
2-s2.0-33646173079
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
The Southeast Asian journal of tropical medicine and public health.. Vol.36 Suppl 4, (2005), 1-4
Suggested Citation
Duangporn Nacapunchai, Chongrak Permmongkol, Sompong Sripochang, Bangourn Sermsart, Thongdee Suvajeejarun Comparison of physiological, cytopathogenic and immunological properties between two environmental isolates of Acanthamoeba spp.. The Southeast Asian journal of tropical medicine and public health.. Vol.36 Suppl 4, (2005), 1-4. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16659
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Comparison of physiological, cytopathogenic and immunological properties between two environmental isolates of Acanthamoeba spp.
Other Contributor(s)
Abstract
The aim of this study was to determine whether pathogenic and less-pathogenic isolates of environmental Acanthamoeba exhibit differences in adhesion to human erythrocytes. Based on physiological properties of temperature, tolerance, and rapid growth, Acanthamoeba were divided into pathogenic and less-pathogenic isolates. Acanthamoeba were tested for their ability to produce cytopathic effects (CPE) using two human cell lines, HEp-2 and KB cells. Both ameba isolates caused CPE to both cell lines with the same pattern without significant difference. Human erythrocytes from 20 healthy volunteers were used to study the erythrocyte reactivity of Acanthamoeba by co-incubation with trophozoites. The pathogenic Acanthamoeba exhibited significantly higher erythrocyte adhesion as compared to the less-pathogens (p<0.05). Erythrocyte activity occurred in the presence of plasma in all blood samples, suggesting the role of plasmatic components and contact-dependent mechanisms to produce host cell cytotoxicity. The present results showed correlation between the physiological properties and erythrocyte reactivity of Acanthamoeba.