Publication: Identification of enterotoxigenic escherichia coli by colony hybridization using three enterotoxin gene probes
Issued Date
1982-01-01
Resource Type
ISSN
15376613
00221899
00221899
Other identifier(s)
2-s2.0-0019977701
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Infectious Diseases. Vol.145, No.6 (1982), 863-869
Suggested Citation
S. L. Moseley, P. Echeverria, J. Seriwatana, C. Tirapat, W. Chaicumpa, T. Sakuldaipeara, S. Falkow Identification of enterotoxigenic escherichia coli by colony hybridization using three enterotoxin gene probes. Journal of Infectious Diseases. Vol.145, No.6 (1982), 863-869. doi:10.1093/infdis/145.6.863 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30334
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Title
Identification of enterotoxigenic escherichia coli by colony hybridization using three enterotoxin gene probes
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Abstract
The applicability of examining clinical specimens with a DNA hybridization technique for genes encoding enterotoxins was examined using enterotoxigenic Escherichia coli (ETEC) that produced both heat-labile toxin (L T) and heat-stable toxin (ST) (24 isolates), ETEC that produced LT only (17 isolates), and ETEC that produced ST only (22 isolates) from Thailand. ETEC was identified with the Y-l adrenal cell and suckling mouse assays. All were homologous with radio labeled fragments of DNA encoding L T or ST of porcine origin (ST-P) or of human origin (ST-H). Strains of ETEC that produced ST only from rural Thailand were homologous with the ST-H probe only, whereas strains isolated in Bangkok were homologous with the ST-H probe, the ST-P probe, or both probes. The hybridization technique detected ETEC in all stool samples of patients with diarrhea from whom ETEC was isolated and in ETEC-inoculated water containing other species of bacteria. The DNA hybridization assay is useful for characterizing and identifying environmental sources of ETEC. © 1982 by the University of Chicago.