Publication:
Rapid Evaluation of Mutant Exon-11 in c-kit in a Recurrent MCT Case Using CD117 Immunocytofluorescence, FACS-Cell Sorting, and PCR

Issued Date

2013-01-01

Resource Type

Article

ISSN

2090701X
20907001

DOI

10.1155/2013/728167

Other identifier(s)

2-s2.0-85048406075

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Case Report in Veterinary Medicine. Vol.2013, (2013)

Suggested Citation

Dettachai Ketpun, Achariya Sailasuta, Prapruddee Piyaviriyakul, Nattawat Onlamoon, Kovit Pattanapanyasat Rapid Evaluation of Mutant Exon-11 in c-kit in a Recurrent MCT Case Using CD117 Immunocytofluorescence, FACS-Cell Sorting, and PCR. Case Report in Veterinary Medicine. Vol.2013, (2013). doi:10.1155/2013/728167 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/32809

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Title

Rapid Evaluation of Mutant Exon-11 in c-kit in a Recurrent MCT Case Using CD117 Immunocytofluorescence, FACS-Cell Sorting, and PCR

Author(s)

Dettachai Ketpun
 Achariya Sailasuta
 Prapruddee Piyaviriyakul
 Nattawat Onlamoon
 Kovit Pattanapanyasat

Other Contributor(s)

Chulalongkorn University
 Mahidol University

Abstract

© 2013 Dettachai Ketpun et al. A 13-year-old, poodle-mixed, male dog was referred to the oncology unit in our faculty's small animal teaching hospital with the problem of rapid recurrent MCT. The owner and the veterinarian would like to use a tyrosine kinase inhibitor (TKI) for the dog. Therefore, fine-needle aspiration (FNA) was performed to collect the MCT cells and these cells were submitted to our laboratory for the detection of internal-tandem-duplicated (ITD) mutation of exon-11 in c-kit, prior to the treatment. The aim of this paper is to demonstrate the use of combinatorial protocol for the rapid evaluation of ITD mutation in MCT cells harvested by FNA. However, there was no ITD-mutant exon-11 that had been observed in this case.

Keyword(s)

Veterinary

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URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/32809

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