Publication: Modification of human cancellous bone using Thai silk fibroin and gelatin for enhanced osteoconductive potential
Issued Date
2013-03-01
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ISSN
15734838
09574530
09574530
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2-s2.0-84876419630
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Materials Science: Materials in Medicine. Vol.24, No.3 (2013), 735-744
Suggested Citation
Rungnapa Vorrapakdee, Sorada Kanokpanont, Juthamas Ratanavaraporn, Saranatra Waikakul, Chris Charoenlap, Siriporn Damrongsakkul Modification of human cancellous bone using Thai silk fibroin and gelatin for enhanced osteoconductive potential. Journal of Materials Science: Materials in Medicine. Vol.24, No.3 (2013), 735-744. doi:10.1007/s10856-012-4830-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31358
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Title
Modification of human cancellous bone using Thai silk fibroin and gelatin for enhanced osteoconductive potential
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Abstract
The modification of human cancellous bone (hBONE) with silk fibroin/gelatin (SF/G) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxysuccini-mide (NHS) crosslinking was established. The SF/G solutions at a weight ratio of 50/50 and the solution concentrations of 1, 2, and 4 wt % were studied. SF/G sub-matrix was formed on the surface and inside pore structure of hBONE. All hBONE scaffolds modified with SF/G showed smaller pore sizes, less porosity, and slightly lower compressive modulus than unmodified hBONE. SF/G sub-matrix was gradually biodegraded in collagenase solution along 4 days. The hBONE scaffolds modified with SF/G, particularly at 2 and 4 wt % solution concentrations, promoted attachment, proliferation, and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (MSC), comparing to the original hBONE. The highest cell number, ALP activity and calcium production were observed for MSC cultured on the hBONE scaffolds modified with 4 wt % SF/G. The mineralization was also remarkably induced in the cases of modified hBONE scaffolds as observed from the deposited calcium phosphate by EDS. The modification of hBONE with SF/G was, therefore, the promising method to enhance the osteoconductive potential of human bone graft for bone tissue engineering. © 2012 Springer Science+Business Media New York.