Publication: Forensic Spermatozoa Detection
Issued Date
2019-12-01
Resource Type
ISSN
1533404X
Other identifier(s)
2-s2.0-85075089447
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
The American journal of forensic medicine and pathology. Vol.40, No.4 (2019), 304-311
Suggested Citation
Papanu Suttipasit Forensic Spermatozoa Detection. The American journal of forensic medicine and pathology. Vol.40, No.4 (2019), 304-311. doi:10.1097/PAF.0000000000000517 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/51255
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Title
Forensic Spermatozoa Detection
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Abstract
Semen is crucial evidence for some sex crimes, with its sole confirmation being sperm detection. The success of sperm detection is dependent on all levels of preanalytic and analytic procedures. Specimen collection must be performed by well-trained and competent forensic physicians as well as forensic nurses, with preservation done properly before laboratory transfer. Laboratory procedures should consider archival sperm identification, by visualization, with adequate amounts separated from other cells to obtain male DNA profiles. Differential extraction is robust and accepted as the forensic standard but is time consuming and may result in male DNA loss. Thus, alternative methods and microdevices have been developed. Challenges in sperm isolation from vaginal or buccal epithelium mixes and discrimination in multiperpetrator cases have been overcome by single-cell profiling; however, problems inherent in identical twin discrimination and azoospermia have yet to be solved. Epigenetics and future molecular biomarkers may hold the key; therefore, all laboratory processes must consider DNA and RNA protection. Long-term specimen preservation should be done when possible in light of future confirmatory tests.