Design sensor chip for repelling the non-specific binding for its application in biomedical sensor

Abstract

© Springer International Publishing Switzerland 2014. The important parameter for the application in biomedical sensor is specificity on the specimen. However, there is the limitation in a low detection signal in real sample applications. The measurement cannot avoid contaminant from some biomolecules, components in patient samples such as blood or serum, which are effect on low detection or error diagnostic. Thus, to decrease this non-specific binding from some contaminants, we designed the sensor chips which are capability on repelling the non-specific binding in order to increase the efficiency of specificity. The ultra thin film and polymeric film were chosen for design. The atomic force microscope (AFM) was used for monitoring behavior of the adsorption of the non-specific binding on the designed chip. The results were found that the ultra thin film can repel some components of samples better than polymeric chip by observation the topography of the sensor chip via AFM. It was found that the area of the covered biomolecules was shown the inhomogeneous and higher roughness in the polymeric thin film than the ultra thin film. It means that there is more residual contaminant from serum on the polymeric chip and less efficiency repelling the contaminant than the results from the ultra thin film. This result was also correlated to measurement the non-specific binding of pateints’ serum via surface Plasmon resonance technique.