Publication: Chromosome abnormalities and absolute telomere lengths of leukocytes from silk weavers with emphasis on potential genotoxicity and mutagenicity of silk dyes
Issued Date
2018-02-01
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ISSN
2476762X
15137368
15137368
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2-s2.0-85042446957
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Cancer Prevention. Vol.19, No.2 (2018), 541-548
Suggested Citation
Onanong Kritpetcharat, Lertchai Charerntanyarak, Usa Lek-Uthai, Peerapol Sukon, Suttiphan Kitcharoen, Panutas Kritpetcharat Chromosome abnormalities and absolute telomere lengths of leukocytes from silk weavers with emphasis on potential genotoxicity and mutagenicity of silk dyes. Asian Pacific Journal of Cancer Prevention. Vol.19, No.2 (2018), 541-548. doi:10.22034/APJCP.2018.19.2.541 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45254
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Title
Chromosome abnormalities and absolute telomere lengths of leukocytes from silk weavers with emphasis on potential genotoxicity and mutagenicity of silk dyes
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Abstract
© 2018, Asian Pacific Organization for Cancer Prevention. Objectives: This study is aimed to assess the possible genotoxicity and mutagenicity of silk dyes on silk weavers. Methods: Peripheral blood leukocytes were obtained from 24 silk weavers and 24 age- and sex-matched controls in northeastern Thailand. After mitogen stimulation in culture, chromosome abnormalities were examined using Giemsa banding and the absolute telomere length (aTL) was measured with SYBR green qRT-PCR. To confirm genotoxic and mutagenic effects of silk dyes, leukocytes from one each of healthy male and female volunteers were cultured with various concentrations of 3 dark red silk dyes under the presence of mitogen. Chromosome abnormalities and the telomere length were determined as above. Results: The proportion of normal metaphase in the silk weaving workers was significantly lower than that in controls. The frequency of chromosome aberrations was higher in the silk weavers than in control group. Polyploidy was detected only in the silk weavers. The aTL was significantly shorter in the silk weavers than in control group (p < 0.05). When leukocytes from normal volunteers were stimulated with mitogen under the presence of various concentrations of 3 silk dyes, suppressed the mitotic index (MI) and normal metaphase, whereas the proportion of prophase and the incomplete chromosome forming increased significantly. All dyes induced polyploidy. Dye #CA5 induced structural changes in male leukocytes, whereas #30 induced the changes in female leukocytes. The #CA5 increased aTL of normal leukocytes in a dose-dependent manner. Conclusions: All dyes, especially #CA5, have high genotoxicity and mutagenicity to induce chromosome aberrations and telomeric instability. Taken all those results together, regular health checking of silk weavers who have been exposed to those dyes is critically necessary to prevent various chemical-induced carcinogenesis.