Publication: Soluble PD-1 rescues the proliferative response of simian immunodeficiency virus-specific CD4 and CD8 T cells during chronic infection
Issued Date
2008-06-01
Resource Type
ISSN
13652567
00192805
00192805
Other identifier(s)
2-s2.0-43749122530
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Immunology. Vol.124, No.2 (2008), 277-293
Suggested Citation
Nattawat Onlamoon, Kenneth Rogers, Ann E. Mayne, Kovit Pattanapanyasat, Kazuyasu Mori, Francois Villinger, Aftab A. Ansari Soluble PD-1 rescues the proliferative response of simian immunodeficiency virus-specific CD4 and CD8 T cells during chronic infection. Immunology. Vol.124, No.2 (2008), 277-293. doi:10.1111/j.1365-2567.2007.02766.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/19326
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Title
Soluble PD-1 rescues the proliferative response of simian immunodeficiency virus-specific CD4 and CD8 T cells during chronic infection
Abstract
Phenotypic and functional studies of the programmed death-1 (PD-1) molecule on CD4+ and CD8+ T cells were performed on peripheral blood mononuclear cells from uninfected and simian immunodeficiency virus (SIV)-infected rhesus macaques. These data demonstrated a rapid upregulation of PD-1 expression on tetramer-positive CD8+ T cells from MamuA.01 + SIV-infected macaques upon infection. Upregulation of PD-1 on total CD8+ T cells was not detectable. In contrast, CD4+ T-cell PD-1 expression was markedly higher in total CD4+ T cells during chronic, but not acute, infection and there was a correlation between the level of PD-1 expression on naive and central memory CD4+ T cells and the levels of viral loads. Such association was emphasized further by a marked decrease of PD-1 expression on tetramer-positive CD8 T cells as well as on CD4+ T cells on longitudinal samples collected before and after the initiation of antiretroviral therapy and downregulation of viral replication in vivo. Cloning of PD-1 and its two ligands from several non-human primate species demonstrated > 95% conservation for PD-1 and PD-L2 and only about 91% homology for PD-L1. Functional studies using soluble recombinant PD-1 protein or PD-1-immunoglobulin G fusion proteins induced marked increases in the SIV-specific proliferative responses of both CD4+ and CD8+ T cells from rhesus macaques. The results of these studies serve as a foundation for future in vivo trials of the use of rMamu-PD-1 to potentially enhance and/or restore antiviral immune responses in vivo. © 2008 The Authors.