Publication: Differential binding with ERα and ERβ of the phytoestrogen-rich plant Pueraria mirifica
Issued Date
2010-01-01
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ISSN
16784510
0100879X
0100879X
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2-s2.0-76649106053
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Mahidol University
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SCOPUS
Bibliographic Citation
Brazilian Journal of Medical and Biological Research. Vol.43, No.2 (2010), 195-200
Suggested Citation
C. Boonchird, T. Mahapanichkul, W. Cherdshewasart Differential binding with ERα and ERβ of the phytoestrogen-rich plant Pueraria mirifica. Brazilian Journal of Medical and Biological Research. Vol.43, No.2 (2010), 195-200. doi:10.1590/S0100-879X2009007500026 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/28820
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Title
Differential binding with ERα and ERβ of the phytoestrogen-rich plant Pueraria mirifica
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Abstract
Variations in the estrogenic activity of the phytoestrogen-rich plant, Pueraria mirifica, were determined with yeast estrogen screen (YES) consisting of human estrogen receptors (hER) hERα and hERβ and human transcriptional intermediary factor 2 (hTIF2) or human steroid receptor coactivator 1 (hSRC1), respectively, together with the β-galactosidase expression cassette. Relative estrogenic potency was expressed by determining the β-galactosidase activity (EC50) of the tuber extracts in relation to 17β-estradiol. Twenty-four and 22 of the plant tuber ethanolic extracts interacted with hERα and hERβ, respectively, with a higher relative estrogenic potency with hERβ than with hERα. Antiestrogenic activity of the plant extracts was also determined by incubation of plant extracts with 17β-estradiol prior to YES assay. The plant extracts tested exhibited antiestrogenic activity. Both the estrogenic and the antiestrogenic activity of the tuber extracts were metabolically activated with the rat liver S9-fraction prior to the assay indicating the positive influence of liver enzymes. Correlation analysis between estrogenic potency and the five major isoflavonoid contents within the previously HPLC-analyzed tuberous samples namely puerarin, daidzin, genistin, daidzein, and genistein revealed a negative result.