Publication: Acute inhibitory effect of alpha-mangostin on sarcoplasmic reticulum calcium-ATPase and myocardial relaxation
Issued Date
2017-10-01
Resource Type
ISSN
10990461
10956670
10956670
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2-s2.0-85020256751
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Biochemical and Molecular Toxicology. Vol.31, No.10 (2017)
Suggested Citation
Sukanya Phungphong, Anusak Kijtawornrat, Pieter P. de Tombe, Jonggonnee Wattanapermpool, Tepmanas Bupha-Intr, Sunit Suksamrarn Acute inhibitory effect of alpha-mangostin on sarcoplasmic reticulum calcium-ATPase and myocardial relaxation. Journal of Biochemical and Molecular Toxicology. Vol.31, No.10 (2017). doi:10.1002/jbt.21942 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/41802
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Title
Acute inhibitory effect of alpha-mangostin on sarcoplasmic reticulum calcium-ATPase and myocardial relaxation
Abstract
© 2017 Wiley Periodicals, Inc. The benefits of α-mangostin for various tissues have been reported, but its effect on the heart has not been clarified. This study aimed to evaluate the effects of α-mangostin on cardiac function. Using a cardiac sarcoplasmic reticulum (SR) membrane preparation, α-mangostin inhibited SR Ca2+-ATPase activity in a dose-dependent manner (IC50of 6.47 ± 0.7 μM). Its suppressive effect was specific to SR Ca2+-ATPase but not to myofibrillar Ca2+-ATPase. Using isolated cardiomyocytes, 50 μM of α-mangostin significantly increased the duration of cell relengthening and increased the duration of Ca2+transient decay, suggesting altered myocyte relaxation. The relaxation effect of α-mangostin was also supported in vivo after intravenous infusion. A significant suppression of both peak pressure and rate of ventricular relaxation (–dP/dt) relative to DMSO infusion was observed. The results from the present study demonstrated that α-mangostin exerts specific inhibitory action on SR Ca2+-ATPase activity, leading to myocardial relaxation dysfunction.