Publication:
Calcineurin subunit B is involved in shell regeneration in Haliotis diversicolor

dc.contributor.authorTiranan Buddawongen_US
dc.contributor.authorSomluk Asuvapongpatanaen_US
dc.contributor.authorChanyatip Suwannasingen_US
dc.contributor.authorValainipha Habuddhaen_US
dc.contributor.authorChompoonut Sukonseten_US
dc.contributor.authorChanyarak Sombutkayasithen_US
dc.contributor.authorCarmel McDougallen_US
dc.contributor.authorWattana Weerachatyanukulen_US
dc.contributor.otherGriffith Universityen_US
dc.contributor.otherWalailak Universityen_US
dc.contributor.otherNaresuan Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2022-08-04T07:59:32Z
dc.date.available2022-08-04T07:59:32Z
dc.date.issued2021-01-12en_US
dc.description.abstractAbalone shells are mainly composed of two major polymorphs of CaCO3 that are distributed in different layers of the shell. The process of shell biomineralization is controlled by genes and proteins expressed within the mantle epithelium. In this present paper, we conducted a shell regeneration experiment to study the role of HcCNA and HcCNB (individual subunits of calcineurin) in shell biomineralization in H. diversicolor. The results of qPCR showed that HcCNB is upregulated to a greater extent than HcCNA in the mantle after shell notching. In vivo study of the effects of rHcCNB injection showed a significantly higher percentage of regenerated shell length, but not area, in the injected group compared to the control group. In addition, SEM observation of the inner surface of the regenerated shells revealed three different zones including prismatic, nacreous, and a distinct transition zone. Changes in the crystal organization and ultrastructure are clearly evident in these three zones, particularly after 3 weeks of rHcCNB administration. We hypothesize that this is due to faster biomineralization rates in the rHcCNB treated group. Taken together, our results demonstrate that HcCNB participates in shell regeneration in H. diversicolor. As calcineurin subunits have also been implicated in shell formation in bivalves, these findings suggest that calcineurin subunits may play important roles in biomineralization in all conchiferans.en_US
dc.identifier.citationPeerJ. Vol.9, (2021)en_US
dc.identifier.doi10.7717/peerj.10662en_US
dc.identifier.issn21678359en_US
dc.identifier.other2-s2.0-85099361519en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/75743
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85099361519&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectNeuroscienceen_US
dc.titleCalcineurin subunit B is involved in shell regeneration in Haliotis diversicoloren_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85099361519&origin=inwarden_US

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