Publication: Molecular detection of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in school children at the Thai-Myanmar border
Issued Date
2021-08-01
Resource Type
ISSN
14321955
09320113
09320113
Other identifier(s)
2-s2.0-85111709843
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology Research. Vol.120, No.8 (2021), 2887-2895
Suggested Citation
Chantira Sutthikornchai, Supaluk Popruk, Aongart Mahittikorn, Dumrongkiet Arthan, Ngamphol Soonthornworasiri, Chirawat Paratthakonkun, Yaoyu Feng, Lihua Xiao Molecular detection of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in school children at the Thai-Myanmar border. Parasitology Research. Vol.120, No.8 (2021), 2887-2895. doi:10.1007/s00436-021-07242-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/75609
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Title
Molecular detection of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in school children at the Thai-Myanmar border
Abstract
Few data are available on the genetic identity of enteric protists Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in humans in Thailand. In this study, 254 stool samples were collected from primary school children from Ratchaburi Province at the Thai-Myanmar border and examined for Cryptosporidium spp., G. duodenalis, E. bieneusi and Cyclospora cayetanensis using PCR techniques. The genotype identity of the pathogens was determined by DNA sequence analysis of the PCR products. Cryptosporidium felis was found in 1 stool sample, G. duodenalis in 19 stool samples, and E. bieneusi in 4 stool samples. For G. duodenalis, sub-assemblage AII was the dominant genotype, but one infection with assemblage F was found. The E. bieneusi genotypes found included known genotypes D and J, and one novel genotype (HPTM1). Cyclospora cayetanensis was not detected in any samples. Results of the preliminary study indicate that children at the Thai-Myanmar border from Ratchaburi Province, Thailand are infected with diverse zoonotic genotypes of Cryptosporidium spp., G. duodenalis, and E. bieneusi.