Publication: Cytogenetic, cross-mating and molecular evidence of four cytological races of Anopheles crawfordi (Diptera: Culicidae) in Thailand and Cambodia
Issued Date
2014-01-01
Resource Type
ISSN
17683238
16310691
16310691
Other identifier(s)
2-s2.0-84908262758
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Comptes Rendus - Biologies. Vol.337, No.11 (2014), 625-634
Suggested Citation
Atiporn Saeung, Visut Baimai, Sorawat Thongsahuan, Yasushi Otsuka, Wichai Srisuka, Kritsana Taai, Pradya Somboon, Wannapa Suwonkerd, Tho Sochanta, Wej Choochote Cytogenetic, cross-mating and molecular evidence of four cytological races of Anopheles crawfordi (Diptera: Culicidae) in Thailand and Cambodia. Comptes Rendus - Biologies. Vol.337, No.11 (2014), 625-634. doi:10.1016/j.crvi.2014.08.001 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/33167
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Cytogenetic, cross-mating and molecular evidence of four cytological races of Anopheles crawfordi (Diptera: Culicidae) in Thailand and Cambodia
Abstract
© 2014 Académie des sciences. Twenty-nine isolines of Anopheles crawfordi were established from wild-caught females collected from cow-baited traps in Thailand and Cambodia. Three types of X (X<inf>1</inf>, X<inf>2</inf>, X<inf>3</inf>) and four types of Y (Y<inf>1</inf>, Y<inf>2</inf>, Y<inf>3</inf>, and Y<inf>4</inf>) chromosomes were identified, according to differing amounts of extra heterochromatin. These sex chromosomes represent four metaphase karyotypes, i.e., Forms A (X<inf>1</inf>, X<inf>2</inf>, X<inf>3</inf>, Y<inf>1</inf>), B (X<inf>1</inf>, X<inf>2</inf>, X<inf>3</inf>, Y<inf>2</inf>), C (X<inf>2</inf>, Y<inf>3</inf>) and D (X<inf>2</inf>, Y<inf>4</inf>). Forms C and D are novel metaphase karyotypes confined to Thailand, whereas forms A and B appear to be common in both Thailand and Cambodia. Cross-mating experiments between the four karyotypic forms indicated genetic compatibility in yielding viable progenies and synaptic salivary gland polytene chromosomes. The results suggest that the forms are conspecific and A. crawfordi comprises four cytological races, which is further supported by very low intraspecific variation (mean genetic distance = 0.000-0.018) of the nucleo.