Publication: Detection of plasmodium falciparum by polymerase chain reaction in a field study
1
Issued Date
1992-01-01
Resource Type
ISSN
15376613
00221899
00221899
Other identifier(s)
2-s2.0-0026719588
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Infectious Diseases. Vol.166, No.1 (1992), 145-148
Suggested Citation
Orntipa Sethabutr, Arthur E. Brown, Sakol Panyim, Kevin C. Kain, H. Kyle Webster, Peter Echeverria Detection of plasmodium falciparum by polymerase chain reaction in a field study. Journal of Infectious Diseases. Vol.166, No.1 (1992), 145-148. doi:10.1093/infdis/166.1.145 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/22313
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Title
Detection of plasmodium falciparum by polymerase chain reaction in a field study
Abstract
Detection of Plasmodium falciparum by polymerase chain reaction (PCR) was evaluated in 33 P. falciparum-infected patients with two different amplification systems over 5-7 days of curative treatment. In the Kl-14 system, a P. falciparum DNA fragment of 206 bp was detected, and in the circumsporozoite (CS) system, a fragment of 800 bp was detected. The Kl-14 and CS systems identified 95% and 93%, respectively, of 103 microscopically identified specimens; both systems detected as few as 11 parasites/*d among these specimens. Specimens from 20 smear- and history-negative controls were all negative by both PCR systems. The Kl-14 and CS systems detected P. falciparum DNA in 53% and 20%, respectively, of blood films collected on the first day and 3% and 0 of the blood films collected on the fourth day after reversion to microscopic negative. The simultaneous use of two independent PCR systems to monitor patients during curative treatment of P. falciparum infections convincingly demonstrated that P. falciparum DNA was present transiently in the blood of infected patients at a time when the parasite could no longer be detected microscopically. © 1992 by the University of Chicago.