Publication: The circulatory small non-coding RNA landscape in community-acquired pneumonia on intensive care unit admission
Issued Date
2021-08-01
Resource Type
ISSN
15821838
Other identifier(s)
2-s2.0-85110295103
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Cellular and Molecular Medicine. Vol.25, No.16 (2021), 7621-7630
Suggested Citation
Hina N. Khan, Aldo Jongejan, Lonneke A. van Vught, Janneke Horn, Marcus J. Schultz, Aeilko H. Zwinderman, Olaf L. Cremer, Marc J. Bonten, Tom van der Poll, Brendon P. Scicluna The circulatory small non-coding RNA landscape in community-acquired pneumonia on intensive care unit admission. Journal of Cellular and Molecular Medicine. Vol.25, No.16 (2021), 7621-7630. doi:10.1111/jcmm.16406 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76093
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
The circulatory small non-coding RNA landscape in community-acquired pneumonia on intensive care unit admission
Other Contributor(s)
Abstract
Community-acquired pneumonia (CAP) is a major cause of sepsis. Despite several clinical trials targeting components of the inflammatory response, no specific treatment other than antimicrobial therapy has been approved. This argued for a deeper understanding of sepsis immunopathology, in particular factors that can modulate the host response. Small non-coding RNA, for example, micro (mi)RNA, have been established as important modifiers of cellular phenotypes. Notably, miRNAs are not exclusive to the intracellular milieu but have also been detected extracellular in the circulation with functional consequences. Here, we sought to determine shifts in circulatory small RNA levels of critically ill patients with CAP-associated sepsis and to determine the influence of clinical severity and causal pathogens on small RNA levels. Blood plasma was collected from 13 critically ill patients with sepsis caused by CAP on intensive care unit admission and from 5 non-infectious control participants. Plasma small RNA-sequencing identified significantly altered levels of primarily mature miRNAs in CAP relative to controls. Pathways analysis of high or low abundance miRNA identified various over-represented cellular biological pathways. Analysis of small RNA levels against common clinical severity and inflammatory parameters indices showed direct and indirect correlations. Additionally, variance of plasma small RNA levels in CAP patients may be explained, at least in part, by differences in causal pathogens. Small nuclear RNA levels were specifically altered in CAP due to Influenza infection in contrast to Streptococcus pneumoniae infection. Pathway analysis of plasma miRNA signatures unique to Influenza or Streptococcus pneumoniae infections showed enrichment for specific proteoglycan, cell cycle, and immunometabolic pathways.