Publication: Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells
Issued Date
2018-07-01
Resource Type
ISSN
18767753
18735061
18735061
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2-s2.0-85047008508
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Mahidol University
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SCOPUS
Bibliographic Citation
Stem Cell Research. Vol.30, (2018), 34-37
Suggested Citation
Bootsakorn Boonkaew, Weeradee Thummavichit, Ratchapong Netsrithong, Chinnavuth Vatanashevanopakorn, Kovit Pattanapanyasat, Methichit Wattanapanitch Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells. Stem Cell Research. Vol.30, (2018), 34-37. doi:10.1016/j.scr.2018.05.002 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/45114
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Title
Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells
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Abstract
© 2018 The Authors Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-derived iPSC line (designated as MUSIi005-A) was karyotypically normal, expressed pluripotent markers, differentiated into cells of three embryonic germ layers, and showed no viral and transgene expressions at passage 29. Our protocol offers a non-invasive and efficient approach for iPSC generation from patients with genetic or acquired disorders.