Publication: Transcriptome analysis of Anopheles dirus and Plasmodium vivax at ookinete and oocyst stages
Issued Date
2020-07-01
Resource Type
ISSN
18736254
0001706X
0001706X
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2-s2.0-85083793591
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Mahidol University
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SCOPUS
Bibliographic Citation
Acta Tropica. Vol.207, (2020)
Suggested Citation
Tippawan Boonkaew, Watcharakorn Mongkol, Sureerat Prasert, Pattaweeya Paochan, Saki Yoneda, Wang Nguitragool, Chalermpon Kumpitak, Jetsumon Sattabongkot, Anchanee Kubera Transcriptome analysis of Anopheles dirus and Plasmodium vivax at ookinete and oocyst stages. Acta Tropica. Vol.207, (2020). doi:10.1016/j.actatropica.2020.105502 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/54424
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Title
Transcriptome analysis of Anopheles dirus and Plasmodium vivax at ookinete and oocyst stages
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Abstract
© 2020 Elsevier B.V. Malaria is transmitted by Plasmodium parasites through the bite of female Anopheles mosquitoes. One of the most important mosquito vectors in the Greater Mekong Subregion is Anopheles dirus. This study reports RNA sequencing (RNA-Seq) transcriptome analysis of An. dirus at 18 hours and 7 days after a P. vivax-infected blood meal, which represent infection at the ookinete and oocyst parasite developmental stages, respectively. Following infection, 582 An. dirus transcripts were modulated. The 2,408 P. vivax transcripts could be classified into ookinete-specific, two-stage, and oocyst-specific groups. Results were validated by quantitative reverse transcription polymerase chain reaction. Gene ontology analysis of the vector and parasite revealed several biological pathways for both, providing a better understanding of Anopheles–Plasmodium interactions at the ookinete and oocyst stages.