Publication: Selection of RNA aptamers that bind HIV-1 LTR DNA duplexes: Strand invaders
Issued Date
2010-12-01
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ISSN
13624962
03051048
03051048
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2-s2.0-78650482160
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Mahidol University
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SCOPUS
Bibliographic Citation
Nucleic Acids Research. Vol.38, No.22 (2010), 8306-8315
Suggested Citation
Chatchawan Srisawat, David R. Engelke Selection of RNA aptamers that bind HIV-1 LTR DNA duplexes: Strand invaders. Nucleic Acids Research. Vol.38, No.22 (2010), 8306-8315. doi:10.1093/nar/gkq696 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28581
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Title
Selection of RNA aptamers that bind HIV-1 LTR DNA duplexes: Strand invaders
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Abstract
RNA that can specifically bind to double-stranded DNA is of interest because it might be used as a means to regulate transcription of the target genes. To explore possible interactions between RNA and duplex DNA, we selected for RNA aptamers that can bind to the long terminal repeats (LTRs) of human immunodeficiency virus type 1 DNA. The selected aptamers were classified into four major groups based on the consensus sequences, which were found to locate in the non-stem regions of the predicted RNA secondary structures, consistent with roles in target binding. Analysis of the aptamer consensus sequences suggested that the conserved segments could form duplexes via Watson-Crick base-pairing with preferred sequences in one strand of the DNA, assuming the aptamer invaded the duplex. The aptamer binding sites on the LTR were experimentally determined to be located preferentially at these sites near the termini of double-stranded target DNA, despite selection schemes that were designed to minimize preferences for termini. The results presented here show that aptamer RNAs can be selected in vitro that strand-invade at preferred DNA duplex sequences to form stable complexes. © 2010 The Author(s).