Publication: LINE-1 methylation in the peripheral blood mononuclear cells of cancer patients
Issued Date
2012-05-18
Resource Type
ISSN
18733492
00098981
00098981
Other identifier(s)
2-s2.0-84858614329
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Mahidol University
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SCOPUS
Bibliographic Citation
Clinica Chimica Acta. Vol.413, No.9-10 (2012), 869-874
Suggested Citation
Nakarin Kitkumthorn, Time Tuangsintanakul, Prakasit Rattanatanyong, Danai Tiwawech, Apiwat Mutirangura LINE-1 methylation in the peripheral blood mononuclear cells of cancer patients. Clinica Chimica Acta. Vol.413, No.9-10 (2012), 869-874. doi:10.1016/j.cca.2012.01.024 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/13729
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Title
LINE-1 methylation in the peripheral blood mononuclear cells of cancer patients
Abstract
Background: Recently, we classified LINE-1 loci according to their methylation statuses and found that the percentage of hypomethylated LINE-1 loci ( u C u C) can differentiate between the peripheral blood mononuclear cells (PBMCs) of oral cancer patients and normal controls with a higher specificity and sensitivity than overall methylation levels. Here, we evaluated the LINE-1 methylation levels and patterns in PBMCs from patients with cancers of the nasopharynx, lung, liver, bile duct, breast and colon. Methods: Combined Bisulfite Restriction Analysis (COBRA) of LINE-1 loci was performed to examine the LINE-1 methylation statuses of PBMCs from 216 cancer patients with 6 different types of cancer compared with 144 normal controls. Results: Only colorectal and nasopharyngeal cancer samples were found to have lower levels of overall LINE-1 methylation compared with normal controls (p < 0.0001 and p=0.0022). However, % u C u C in cancers of the colon, liver, lung and nasopharynx was significantly higher compared with normal controls (p < 0.0001, p < 0.0001, p=0.01 and p=0.001, respectively). Furthermore, ROC curve analyses of these four cancer types also demonstrated the potential of % u C u C as a biomarker for cancer diagnosis. Conclusion: Changes in th e levels and patterns of genome-wide methylation of PBMCs are associated with cancer risk. For LINE-1, % u C u C is a more effective tumour marker for determining cancer risk than overall methylation levels. © 2012 Elsevier B.V.