Publication: Automated cytochemistry in non-Hodgkin's lymphoma: A new method for determination of cells from lymph node biopsy
Issued Date
1986-01-01
Resource Type
ISSN
14219662
00015792
00015792
Other identifier(s)
2-s2.0-0022974624
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Acta Haematologica. Vol.75, No.4 (1986), 199-202
Suggested Citation
Ahnond Bunyaratvej, Pattraporn Boonkanta, Prawat Nitiyanant, Suntaree Apibal, Natth Bhamarapravati Automated cytochemistry in non-Hodgkin's lymphoma: A new method for determination of cells from lymph node biopsy. Acta Haematologica. Vol.75, No.4 (1986), 199-202. doi:10.1159/000206124 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/9829
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Automated cytochemistry in non-Hodgkin's lymphoma: A new method for determination of cells from lymph node biopsy
Other Contributor(s)
Abstract
Cell suspension prepared from the lymph node biopsy of patients with non-Hodgkin's lymphoma (NHL), metastatic carcinoma (MC) and non-neoplastic lymphadenopathies (NL) were analyzed by the Hemalog D, automated differential counter. The preparation of lymph node cells is described first in this study. The results show that the percentage of large cells (diameter greater than 13.5 μm) stained negatively with peroxidase (LUC, large unstained cells) was remarkably increased in patients with NHL (mean ± SEM = 18.6 ± 3.1 %) and was particularly increased in the subgroup, diffuse histiocytic type (31.1 ± 5.3 %). Patients with MC had a raised percentage of nonspecific esterase-positive cells (9.2 ± 1.4%) compared to patients in the NHL and NL groups. Patients in the NL group had low percentages of both LUC (5.3 ± 0.7%) and nonspecific esterase-positive cells (1.8 ± 0.2%). Quantitation of cells in the lymph node by using the Hemalog D may assist in the diagnosis of lymph node diseases. © 1986 S. Karger AG, Basel.