Publication: Bioactive peptide isolated from sesame seeds inhibits cell proliferation and induces apoptosis and autophagy in leukemic cells
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Issued Date
2021-01-01
Resource Type
ISSN
16112156
Other identifier(s)
2-s2.0-85103858279
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Mahidol University
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SCOPUS
Bibliographic Citation
EXCLI Journal. Vol.20, (2021), 709-721
Suggested Citation
Kamolchanok Deesrisak, Yodying Yingchutrakul, Sucheewin Krobthong, Sittiruk Roytrakul, Chawalit Chatupheeraphat, Paweena Subkorn, Usanarat Anurathapan, Dalina Tanyong Bioactive peptide isolated from sesame seeds inhibits cell proliferation and induces apoptosis and autophagy in leukemic cells. EXCLI Journal. Vol.20, (2021), 709-721. doi:10.17179/excli2021-3406 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/75790
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Title
Bioactive peptide isolated from sesame seeds inhibits cell proliferation and induces apoptosis and autophagy in leukemic cells
Abstract
Leukemia is the most common type of hematological malignancies. Several natural products including bioactive peptides have been explored and studied for their anti-leukemic activities. In the present study, anti-leukemic peptide, IGTLILM (IM-7), was isolated and identified from the protein hydrolysate of sesame seeds by reverse phase-solid phase extraction, off-gel fractionation and nano LC-MS/MS. The cytotoxic effects of IM-7 were studied in MOLT-4 and NB4 acute leukemic cell lines using an MTT assay. The induction of apoptosis and autophagy was investigated by flow cytometry using Annexin V-FITC/PI staining and anti-LC3/FITC antibodies, respectively. The mRNA alterations of apoptotic and autophagic-related genes were determined by reverse transcriptionquantitative PCR. The present study found that IM-7 inhibited the proliferation of MOLT-4 and NB4 cells in dosedependent manner, but it showed a minimal effect on healthy mononuclear cells. IM-7 activated apoptosis and autophagy through the upregulation of CASP3, ULK1 and BECN1 and the downregulation of BCL2. In addition, IM-7 enhanced the cytotoxic effect of the anti-leukemic drug, daunorubicin. The findings suggested that IM-7 was potent to suppress the proliferation of MOLT-4 and NB4 leukemic cells and induce apoptosis and autophagy through the regulation of caspase 3-Bcl-2 and ULK1-Beclin1, respectively.