Publication: Soluble granzymes are released during human endotoxemia and in patients with severe infection due to gram-negative bacteria
Issued Date
2000-08-12
Resource Type
ISSN
00221899
DOI
Other identifier(s)
2-s2.0-0033914223
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Infectious Diseases. Vol.182, No.1 (2000), 206-213
Suggested Citation
Fanny N. Lauw, Andrew J.H. Simpson, C. Erik Hack, Jan M. Prins, Angela M. Wolbink, Sander J.H. Van Deventer, Wipada Chaowagul, Nicholas J. White, Tom Van Der Poll Soluble granzymes are released during human endotoxemia and in patients with severe infection due to gram-negative bacteria. Journal of Infectious Diseases. Vol.182, No.1 (2000), 206-213. doi:10.1086/315642 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/26193
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Title
Soluble granzymes are released during human endotoxemia and in patients with severe infection due to gram-negative bacteria
Abstract
Extracellular release of granzymes is considered to reflect the involvement of cytotoxic T lymphocytes and NK cells in various disease states. To obtain insight into granzyme release during bacterial infection, granzyme levels were measured during experimental human endotoxemia and in patients with melioidosis, a severe infection due to gram-negative bacteria. Plasma concentrations of granzyme A (GrA) and GrB increased transiently after endotoxin administration, peaking after 2-6 h. In patients with bacteremic melioidosis, GrA and GrB levels were elevated on admission and remained high during the 72-h study period. In whole blood stimulated with heat-killed Burkholderia pseudomallei, neutralization of tumor necrosis factor, interleukin-12, or interleukin-18 inhibited granzyme secretion, which was independent of interferon-γ. Stimulation with endotoxin and other gram- negative and gram-positive bacteria also strongly induced the secretion of granzymes, suggesting that granzyme release is a general immune response during bacterial infection. The interaction between the cytokine network and granzymes may play an important immunoregulatory role during bacterial infections.