Publication: Free radical scavenging and anti-acne activities of mangosteen fruit rind extracts prepared by different extraction methods
Issued Date
2010-02-12
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ISSN
17445116
13880209
13880209
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2-s2.0-76149083889
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Mahidol University
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SCOPUS
Bibliographic Citation
Pharmaceutical Biology. Vol.48, No.2 (2010), 182-186
Suggested Citation
Werayut Pothitirat, Mullika Traidej Chomnawang, Roongtawan Supabphol, Wandee Gritsanapan Free radical scavenging and anti-acne activities of mangosteen fruit rind extracts prepared by different extraction methods. Pharmaceutical Biology. Vol.48, No.2 (2010), 182-186. doi:10.3109/13880200903062671 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/28782
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Title
Free radical scavenging and anti-acne activities of mangosteen fruit rind extracts prepared by different extraction methods
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Abstract
The ethanol extracts of mangosteen fruit rinds prepared by several extraction methods were examined for their contents of bioactive compounds, DPPH-scavenging activity, and anti-acne producing bacteria against Propionibacterium acnes and Staphylococcus epidermidis. The dried powder of the fruit rind was extracted with 95% ethanol by maceration, percolation, Soxhlet extraction, ultrasonic extraction, and extraction using a magnetic stirrer. Soxhlet extraction promoted the maximum contents of crude extract (26.60% dry weight) and α-mangostin (13.51%, w/w of crude extract), and also gave the highest anti-acne activity with MIC 7.81 and 15.63μg/mL and MBC 15.53 and 31.25μg/mL against P. acnes and S. epidermidis, respectively. Ethanol 70% and 50% (v/v) were also compared in Soxhlet extraction. Ethanol 50% promoted the extract with maximum amounts of total phenolic compounds (26.96g gallic acid equivalents/100g extract) and total tannins (46.83g tannic acid equivalents/100g extract), and also exhibited the most effective DPPH-scavenging activity (EC5012.84μg/mL). Considering various factors involved in the process, Soxhlet extraction carried a low cost in terms of reagents and extraction time. It appears to be the recommended extraction method for mangosteen fruit rind. Ethanol 50% should be the appropriate solvent for extracting free radical-scavenging components, phenolic compounds, and tannins, while 95% ethanol is recommended for extraction of α-mangostin, a major anti-acne component from this plant. © 2010 Informa UK Ltd.