Publication: Molecular and functional characterization of vacuolar-ATPase from the American dog tick Dermacentor variabilis
1
Issued Date
2014-02-01
Resource Type
ISSN
13652583
09621075
09621075
Other identifier(s)
2-s2.0-84891735481
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Insect Molecular Biology. Vol.23, No.1 (2014), 42-51
Suggested Citation
N. Petchampai, P. Sunyakumthorn, M. L. Guillotte, C. Thepparit, M. T. Kearney, A. Mulenga, A. F. Azad, K. R. MacAluso Molecular and functional characterization of vacuolar-ATPase from the American dog tick Dermacentor variabilis. Insect Molecular Biology. Vol.23, No.1 (2014), 42-51. doi:10.1111/imb.12059 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/33062
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Molecular and functional characterization of vacuolar-ATPase from the American dog tick Dermacentor variabilis
Abstract
Vacuolar (V)-ATPase is a proton-translocating enzyme that acidifies cellular compartments for various functions such as receptor-mediated endocytosis, intracellular trafficking and protein degradation. Previous studies in Dermacentor variabilis chronically infected with Rickettsia montanensis have identified V-ATPase as one of the tick-derived molecules transcribed in response to rickettsial infection. To examine the role of the tick V-ATPase in tick-Rickettsia interactions, a full-length 2887-bp cDNA (2532-bp open reading frame) clone corresponding to the transcript of the V0 domain subunit a of D. variabilisâ€...V-ATPase (DvVATPaseV0a) gene encoding an 843 amino acid protein with an estimated molecular weight of ∼96 kDa was isolated from D. variabilis. Amino acid sequence analysis of DvVATPaseV0a showed the highest similarity to VATPaseV0a from Ixodes scapularis. A potential N-glycosylation site and eight putative transmembrane segments were identified in the sequence. Western blot analysis of tick tissues probed with polyclonal antibody raised against recombinant DvVATPaseV0a revealed the expression of V-ATPase in the tick ovary. Transcriptional profiles of DvVATPaseV0a demonstrated a greater mRNA expression in the tick ovary, compared with the midgut and salivary glands; however, the mRNA level in each of these tick tissues remained unchanged after infection with R. montanensis for 1 h. V-ATPase inhibition bioassays resulted in a significant decrease in the ability of R. montanensis to invade tick cells in vitro, suggesting a role of V-ATPase in rickettsial infection of tick cells. Characterization of tick-derived molecules involved in rickettsial infection is essential for a thorough understanding of rickettsial transmission within tick populations and the ecology of tick-borne rickettsial diseases. © 2013 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of The Royal Entomological Society.