Publication:
The PneuCarriage Project: A Multi-Centre Comparative Study to Identify the Best Serotyping Methods for Examining Pneumococcal Carriage in Vaccine Evaluation Studies

Issued Date

2015-11-01

Resource Type

Article

ISSN

15491676
15491277

DOI

10.1371/journal.pmed.1001903

Other identifier(s)

2-s2.0-85000501013

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

PLoS Medicine. Vol.12, No.11 (2015)

Suggested Citation

Catherine Satzke, Eileen M. Dunne, Barbara D. Porter, Keith P. Klugman, E. Kim Mulholland, Jorge E. Vidal, Fuminori Sakai, Janet E. Strachan, Deborah C. Hay Burgess, Douglas Holtzman, K. Boelsen, Maha Habib, Jayne Manning, Belinda D. Ortika, Casey L. Pell, Jenna A. Smyth, Martin Antonio, Keith P. Klugman, Katherine L. O’Brien, Roy M. Robins-Browne, J. Anthony Scott, Samir K. Saha, Fiona M. Russell, Andrew R. Greenhill, Deborah Lehmann, Peter V. Adrian, Shabir A. Madhi, Lorry G. Rubin, Atqia Rizvi, Jason Hinds, Katherine A. Gould, Fanrong Kong, Shahin Oftadeh, Gwendolyn L. Gilbert, Lu Feng, Boyang Cao, Gláucia Paranhos-Baccalà, Jean Noel Telles, Mélina Messaoudi, Ray Borrow, Elaine Stanford, Robert George, Carmen Sheppard, Silvio D. Brugger, Kathrin Mühlemann, Markus Hilty, Ismar A. Rivera-Olivero, Jacobus H. de Waard, Bambos M. Charalambous, Marcus H. Leung, Chiara Azzari, Maria Moriondo, Francesco Nieddu, Peter W.M. Hermans, Christa E. van der Gaast-de Jongh, Paul Turner, David J. Ecker, Rangarajan Sampath The PneuCarriage Project: A Multi-Centre Comparative Study to Identify the Best Serotyping Methods for Examining Pneumococcal Carriage in Vaccine Evaluation Studies. PLoS Medicine. Vol.12, No.11 (2015). doi:10.1371/journal.pmed.1001903 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/36279

Research Projects

Organizational Units

Authors

Journal Issue

Thesis

Title

The PneuCarriage Project: A Multi-Centre Comparative Study to Identify the Best Serotyping Methods for Examining Pneumococcal Carriage in Vaccine Evaluation Studies

Author(s)

Catherine Satzke
 Eileen M. Dunne
 Barbara D. Porter
 Keith P. Klugman
 E. Kim Mulholland
 Jorge E. Vidal
 Fuminori Sakai
 Janet E. Strachan
 Deborah C. Hay Burgess
 Douglas Holtzman
 K. Boelsen
 Maha Habib
 Jayne Manning
 Belinda D. Ortika
 Casey L. Pell
 Jenna A. Smyth
 Martin Antonio
 Keith P. Klugman
 Katherine L. O’Brien
 Roy M. Robins-Browne
 J. Anthony Scott
 Samir K. Saha
 Fiona M. Russell
 Andrew R. Greenhill
 Deborah Lehmann
 Peter V. Adrian
 Shabir A. Madhi
 Lorry G. Rubin
 Atqia Rizvi
 Jason Hinds
 Katherine A. Gould
 Fanrong Kong
 Shahin Oftadeh
 Gwendolyn L. Gilbert
 Lu Feng
 Boyang Cao
 Gláucia Paranhos-Baccalà
 Jean Noel Telles
 Mélina Messaoudi
 Ray Borrow
 Elaine Stanford
 Robert George
 Carmen Sheppard
 Silvio D. Brugger
 Kathrin Mühlemann
 Markus Hilty
 Ismar A. Rivera-Olivero
 Jacobus H. de Waard
 Bambos M. Charalambous
 Marcus H. Leung
 Chiara Azzari
 Maria Moriondo
 Francesco Nieddu
 Peter W.M. Hermans
 Christa E. van der Gaast-de Jongh
 Paul Turner
 David J. Ecker
 Rangarajan Sampath

Other Contributor(s)

Royal Children's Hospital, Melbourne
 University of Melbourne
 Rollins School of Public Health
 London School of Hygiene & Tropical Medicine
 Bill and Melinda Gates Foundation
 Kimataifa Diagnostics & Devices Consulting
 Takeda Vaccines
 Murdoch Children's Research Institute
 Vaccinology Theme
 Johns Hopkins Bloomberg School of Public Health
 Kenya Medical Research Institute
 Dhaka Shishu Hospital
 Papua New Guinea Institute of Medical Research
 Federation University Australia
 Telethon Kids Institute
 University of Witwatersrand
 Children's Medical Center
 University of London
 Westmead Hospital
 The University of Sydney
 Nankai University
 Public Health England
 Statens Serum Institut
 University of Bern
 The Forsyth Institute
 Harvard School of Dental Medicine
 UniversitatsSpital Bern
 Universidad Central de Venezuela
 UCL
 City University of Hong Kong
 Universita degli Studi di Firenze
 Radboud University Nijmegen Medical Centre
 Mahidol University
 Angkor Hospital for Children
 University of Oxford

Abstract

© 2015 Satzke et al. Background: The pneumococcus is a diverse pathogen whose primary niche is the nasopharynx. Over 90 different serotypes exist, and nasopharyngeal carriage of multiple serotypes is common. Understanding pneumococcal carriage is essential for evaluating the impact of pneumococcal vaccines. Traditional serotyping methods are cumbersome and insufficient for detecting multiple serotype carriage, and there are few data comparing the new methods that have been developed over the past decade. We established the PneuCarriage project, a large, international multi-centre study dedicated to the identification of the best pneumococcal serotyping methods for carriage studies. Methods and Findings: Reference sample sets were distributed to 15 research groups for blinded testing. Twenty pneumococcal serotyping methods were used to test 81 laboratory-prepared (spiked) samples. The five top-performing methods were used to test 260 nasopharyngeal (field) samples collected from children in six high-burden countries. Sensitivity and positive predictive value (PPV) were determined for the test methods and the reference method (traditional serotyping of >100 colonies from each sample). For the alternate serotyping methods, the overall sensitivity ranged from 1% to 99% (reference method 98%), and PPV from 8% to 100% (reference method 100%), when testing the spiked samples. Fifteen methods had ≥70% sensitivity to detect the dominant (major) serotype, whilst only eight methods had ≥70% sensitivity to detect minor serotypes. For the field samples, the overall sensitivity ranged from 74.2% to 95.8% (reference method 93.8%), and PPV from 82.2% to 96.4% (reference method 99.6%). The microarray had the highest sensitivity (95.8%) and high PPV (93.7%). The major limitation of this study is that not all of the available alternative serotyping methods were included. Conclusions: Most methods were able to detect the dominant serotype in a sample, but many performed poorly in detecting the minor serotype populations. Microarray with a culture amplification step was the top-performing method. Results from this comprehensive evaluation will inform future vaccine evaluation and impact studies, particularly in low-income settings, where pneumococcal disease burden remains high.

Keyword(s)

Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/36279

Collections

Scopus 2011-2015