Publication: Acrylonitrile induced apoptosis via oxidative stress in neuroblastoma SH-SY5Y cell
Issued Date
2010-10-01
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ISSN
10991263
0260437X
0260437X
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2-s2.0-78049268143
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Applied Toxicology. Vol.30, No.7 (2010), 649-655
Suggested Citation
Piyajit Watcharasit, Sumitra Suntararuks, Daranee Visitnonthachai, Apinya Thiantanawat, Jutamaad Satayavivad Acrylonitrile induced apoptosis via oxidative stress in neuroblastoma SH-SY5Y cell. Journal of Applied Toxicology. Vol.30, No.7 (2010), 649-655. doi:10.1002/jat.1535 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/29915
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Title
Acrylonitrile induced apoptosis via oxidative stress in neuroblastoma SH-SY5Y cell
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Abstract
Acrylonitrile (ACN) is a chemical that is widely used in the production of plastics, acrylic fibers, synthetic rubbers and resins. It has been reported that ACN can cause oxidative stress, a condition which is well recognized as an apoptotic initiator; however, information regarding ACN-induced apoptosis is limited. This present study investigated whether ACN induces apoptosis in human neuroblastoma SH-SY5Y cells, and whether its apoptotic induction involves oxidative stress. The results showed that ACN caused activation of caspase-3, a key enzyme involved in apoptosis, in a dose- and time-dependent manner. Detection of sub-G1 apoptotic cell death and apoptotic nuclear condensation revealed that ACN caused an increase in the number of apoptotic cells indicating ACN induces apoptosis in SH-SY5Y cells. ACN dose- and time-dependently increased the level of proapoptotic protein, Bax. Pretreatment with N-acetylcysteine (NAC), an antioxidant, attenuated caspase-3 activation by ACN, as evidenced by a reduction in proteolysis of PARP, a known caspase-3 substrate, as well as in the number of sub-G1 apoptotic cells. Moreover, induction of Bax by ACN was abolished by NAC. Taken together, the results indicate that ACN induces apoptosis in SH-SY5Y cells via a mechanism involving generation of oxidative stress-mediated Bax induction. Copyright © 2010 John Wiley & Sons, Ltd.