Publication: 3-Hydroxy-3-methylglutaryl coenzyme a reductase from the latex of Hevea brasiliensis
Issued Date
1990-01-01
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ISSN
00319422
Other identifier(s)
2-s2.0-0011822416
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Mahidol University
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SCOPUS
Bibliographic Citation
Phytochemistry. Vol.29, No.5 (1990), 1401-1403
Suggested Citation
Rapepun Wititsuwannakul, Dhirayos Wititsuwannakul, Plueng Suwanmanee 3-Hydroxy-3-methylglutaryl coenzyme a reductase from the latex of Hevea brasiliensis. Phytochemistry. Vol.29, No.5 (1990), 1401-1403. doi:10.1016/0031-9422(90)80089-Y Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15899
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Title
3-Hydroxy-3-methylglutaryl coenzyme a reductase from the latex of Hevea brasiliensis
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Abstract
3-Hydroxy-3-methylglutaryl coenzyme A reductase (mevalonate: NADP + oxidoreductase acylating CoA; EC 1.1.1.34) was purified from fresh Hevea latex of clone RRIM 600. The latex was centrifuged and the sediment used for enzyme purification. The enzyme was solubilized by freeze-thawing in a buffer containing 1 % Brij W-1 and 20% glycerol. Affinity chromatography (HMG-CoA-Hexyl-Agarose) was used in the final purification step. The M r determined by SDS-PAGE was 44000 and that estimated from non-denaturing gel electrophoresis, 176000. The optimal pH was ca 7, with an apparent K m of 13 μM for (S)-HMG-CoA. A low concentration of dithiothreitol was required for maximal activity of the purified enzyme. © 1990.