Publication:
Electron microscopic and biochemical analyses of the organization of human sperm chromatin decondensed with Sarkosyl and dithiothreitol

Issued Date

1982-01-01

Resource Type

Article

ISSN

1097010X
0022104X

DOI

10.1002/jez.1402230309

Other identifier(s)

2-s2.0-0020209963

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Journal of Experimental Zoology. Vol.223, No.3 (1982), 277-290

Suggested Citation

Prasert Sobhon, Nongnuj Tanphaichitr, Chutima Chutatape, Padung Vongpayabal, Wannana Panuwatsuk Electron microscopic and biochemical analyses of the organization of human sperm chromatin decondensed with Sarkosyl and dithiothreitol. Journal of Experimental Zoology. Vol.223, No.3 (1982), 277-290. doi:10.1002/jez.1402230309 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30283

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Title

Electron microscopic and biochemical analyses of the organization of human sperm chromatin decondensed with Sarkosyl and dithiothreitol

Author(s)

Prasert Sobhon
 Nongnuj Tanphaichitr
 Chutima Chutatape
 Padung Vongpayabal
 Wannana Panuwatsuk

Other Contributor(s)

Mahidol University
 Harvard Medical School

Abstract

Human sperm chromatin was decondensed by treating the purified sperm heads with Sarkosyl for 60 minutes followed with dithiothreitol (DTT) for 20 minutes and overnight. Following Sarkosyl treatment all histones and nonhistones were removed; the remaining nucleoprotamines in the sperm heads exhibited two levels of higher‐order structure in the forms of 900–1200 Å thick and 380–520 Å thin knobby cords, which were randomly coiled. Subsequent treatment with DTT resulted in the dissociation of the 380–520 Å cords into subunits of 180–210 Å fibers, which were further decondensed into beads‐on‐a‐string structure with diameter of the beads about 120–150 Å. Copyright © 1982 Wiley‐Liss, Inc., A Wiley Company

Keyword(s)

Agricultural and Biological Sciences

Availability

URI

https://repository.li.mahidol.ac.th/handle/20.500.14594/30283

Collections

Scopus 1969-1990