Publication: Determination of appropriate cryopreservation protocols for epididymal cat spermatozoa
3
Issued Date
2015-01-01
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ISSN
14390531
09366768
09366768
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2-s2.0-84928266645
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Mahidol University
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SCOPUS
Bibliographic Citation
Reproduction in Domestic Animals. Vol.50, No.3 (2015), 378-385
Suggested Citation
K. Buranaamnuay Determination of appropriate cryopreservation protocols for epididymal cat spermatozoa. Reproduction in Domestic Animals. Vol.50, No.3 (2015), 378-385. doi:10.1111/rda.12496 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/35218
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Title
Determination of appropriate cryopreservation protocols for epididymal cat spermatozoa
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Abstract
© 2015 Blackwell Verlag GmbH. Effects of Equex and glycerol additions and sample dilution step on frozen-thawed epididymal cat spermatozoa were investigated. The epididymal sperm pellets were resuspended in extenders using one- (groups III and IV) or two- (groups I, II, V and VI) step dilution. For one-step dilution, the pellets were resuspended in plain egg yolk-Tris medium (EYT) + 5% glycerol with (IV)/without (III) 0.5% Equex and cooled (4<sup>°</sup>C, 1 h). For two-step dilution, the pellets were resuspended in EYT (I and V) and in EYT + 3% glycerol (II and VI), cooled and further diluted with EYT + 10% glycerol with (I)/without (V) 1% Equex and with EYT + 7% glycerol with (II)/without (VI) 1% Equex. Immediately after freeze-thawing, no differences (p > 0.05) were found in the motility, viability and membrane integrity (HOST) among the groups except the lowest HOST in IV (p = 0.005 to p = 0.04). The acrosome integrity (FITC) in group I was comparable to that in group II (p > 0.05) and was higher than the rest (p < 0.001 to p = 0.02). At 2 h after thawing, the motility, viability and HOST were comparable among the groups (p > 0.05) except the lower percentages of viability in III (p = 0.008 to p = 0.3) and of HOST in IV (p = 0.005 to p = 0.2). Two-step dilutions with Equex (I, II) were more beneficial for the FITC at 2 h than without Equex (V) (p = 0.005 and p = 0.02) and than one-step dilutions (III, IV) (p < 0.001 to p = 0.02). In conclusion, epididymal cat sperm quality after freeze-thawing could be improved when Equex was added and two-step dilution was performed during freezing. The extenders prepared for the first step of dilution could be with (3%) or without (0%) glycerol.