Publication: Plasmodium falciparum: Effect of anti-malarial drugs on the production and secretion characteristics of histidine-rich protein II
Issued Date
2002-11-01
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ISSN
00144894
Other identifier(s)
2-s2.0-0038731006
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Mahidol University
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SCOPUS
Bibliographic Citation
Experimental Parasitology. Vol.102, No.3-4 (2002), 157-163
Suggested Citation
Harald Noedl, Chansuda Wongsrichanalai, Robert Scott Miller, Khin Saw Aye Myint, Sornchai Looareesuwan, Yaowalark Sukthana, Varee Wongchotigul, Herwig Kollaritsch, Gerhard Wiedermann, Walther H. Wernsdorfer Plasmodium falciparum: Effect of anti-malarial drugs on the production and secretion characteristics of histidine-rich protein II. Experimental Parasitology. Vol.102, No.3-4 (2002), 157-163. doi:10.1016/S0014-4894(03)00051-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20189
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Title
Plasmodium falciparum: Effect of anti-malarial drugs on the production and secretion characteristics of histidine-rich protein II
Abstract
Plasmodium falciparum histidine-rich protein II (HRP2) is one of the best documented malaria proteins. However, little is known about the development of HRP2 concentrations under the influence of anti-malarial drugs. HRP2 levels were determined in cell medium mixture, cellular compartment, and in culture supernatant using a double-site sandwich ELISA specific for HRP2. Characteristic increases in the overall HRP2 levels were found during the later ring and the trophozoite stages. Throughout the later schizont development, rupture, and reinvasion, however, the HRP2 levels remained comparatively stable. When the cultures were exposed to serial dilutions of anti-malarial drugs, a distinct inhibition of HRP2 production was seen with increasing concentrations of drugs, resulting in sigmoid dose-response curves, similar to those obtained from conventional drug sensitivity assays. HRP2 therefore allows for a very accurate estimation of parasite development and its inhibition and may therefore be ideally suited for use in drug sensitivity or bioassays. © 2003 Elsevier Science (USA). All rights reserved.