Publication: Extraction method for high free radical scavenging activity of Siamese neem tree flowers
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Issued Date
2009-10-21
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ISSN
01253395
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2-s2.0-70350012046
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Mahidol University
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SCOPUS
Bibliographic Citation
Songklanakarin Journal of Science and Technology. Vol.31, No.4 (2009), 419-423
Suggested Citation
Worarat Chaisawangwong, Wandee Gritsanapan Extraction method for high free radical scavenging activity of Siamese neem tree flowers. Songklanakarin Journal of Science and Technology. Vol.31, No.4 (2009), 419-423. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/28382
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Title
Extraction method for high free radical scavenging activity of Siamese neem tree flowers
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Abstract
Siamese neem tree (Azadirachta indica A. Juss. var. siamensis Valeton) is a medicinal plant found in Thailand. Young leaves and young flowers of this plant are commonly consumed as a bitter tonic vegetable. The flowers are also used for treatment of fever. The flower extract has been reported to exhibit in vitro free radical scavenging activity and can inhibit lipid peroxidation of bronchogenic cancer cell line. Active compounds in the flowers are flavonoids such as rutin and quercetin. The content of these compounds in the crude extract depends on the method of extraction. Therefore, the appropriate extraction method promoting high yield of total flavonoids and high free radical scavenging activity was investigated in this study. Six different extraction methods, i.e. maceration, percolation, decoction, soxhlet extraction, ultrasonic extraction (UE), and microwave assisted extraction (MA) were carried out for extracting dried powder of Siamese neem tree young flowers. The solvent used for maceration, percolation, and soxhlet extraction was 50% ethanol, while distilled water was used for decoction and MA, and both solvents were used for UE. The content of crude extract, free radical scavenging activity, and total flavonoids content of each extract were investigated and compared. Comparing the various extraction methods, decoction provided an extract containing a high amount of total flavonoids (17.54 mgRE/g extract) and promoting the highest scavenging activity at EC50 11.36 μg/ml. Decoction is also simple, cheap, and convenient and could be used in developing countries. Thus, it should be the recommended extraction method for the flowers of Siamese neem tree for further development of antioxidant pharmaceutical preparations.