Publication: Identification and characterization of the Plasmodium falciparum RhopH2 ortholog in Plasmodium vivax
Issued Date
2013-02-01
Resource Type
ISSN
14321955
09320113
09320113
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2-s2.0-84878350642
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Mahidol University
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SCOPUS
Bibliographic Citation
Parasitology Research. Vol.112, No.2 (2013), 585-593
Suggested Citation
Bo Wang, Feng Lu, Yang Cheng, Jian Li, Daisuke Ito, Jetsumon Sattabongkot, Takafumi Tsuboi, Eun Taek Han Identification and characterization of the Plasmodium falciparum RhopH2 ortholog in Plasmodium vivax. Parasitology Research. Vol.112, No.2 (2013), 585-593. doi:10.1007/s00436-012-3170-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31072
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Title
Identification and characterization of the Plasmodium falciparum RhopH2 ortholog in Plasmodium vivax
Abstract
Plasmodium vivax is one of the most important human malaria species that is geographically widely endemic and potentially affects a larger number of people than its more notorious cousin, Plasmodium falciparum. During invasion of red blood cells, the parasite requires the intervention of high molecular weight complex rhoptry proteins (RhopH) that are also essential for cytoadherence. PfRhopH2, a member of the RhopH multigene family, has been characterized as being crucial during P. falciparum infection. This study describes identifying and characterizing the pfrhoph2 orthologous gene in P. vivax (hereinafter named pvrhoph2). The PvRhopH2 is a 1,369-amino acid polypeptide encoded by PVX-099930 gene, for which orthologous genes have been identified in other Plasmodium species by bioinformatic approaches. Both P. falciparum and P. vivax genes contain nine introns, and there is a high degree of similarity between the deduced amino acid sequences of the two proteins. Moreover, PvRhopH2 contains a signal peptide at its N-terminus and 12 cysteines predominantly in its C-terminal half. PvRhopH2 is localized in one of the apical organelles of the merozoite, the rhoptry, and the localization pattern is similar to that of PfRhopH2 in P. falciparum. The recombinant PvRhopH2 protein is recognized by serum antibodies of patients naturally exposed to P. vivax, suggesting that PvRhopH2 is immunogenic in humans. © 2012 Springer-Verlag Berlin Heidelberg.