Publication: Isolation and characterization of a novel podovirus which infects burkholderia pseudomallei
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Issued Date
2011
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eng
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Mahidol University
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BioMed Central
Bibliographic Citation
Virology Journal. Vol. 8, (2011), 366
Suggested Citation
Jiraporn Gatedee, Kanyanan Kritsiriwuthinan, Galyov, Edouard E., Jinyu Shan, Dubinina, Elena, Narin Intarak, Clokie, Martha RJ. Isolation and characterization of a novel podovirus which infects burkholderia pseudomallei. Virology Journal. Vol. 8, (2011), 366. doi:10.1186/1743-422X-8-366 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/2637
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Title
Isolation and characterization of a novel podovirus which infects burkholderia pseudomallei
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Abstract
Burkholderia pseudomallei is a saprophytic soil bacterium and the etiological agent that causes melioidosis. It is
naturally resistant to many antibiotics and therefore is difficult to treat. Bacteriophages may provide an alternative
source of treatment. We have isolated and characterised the bacteriophage FBp-AMP1. The phage is a member of
the Podoviridae family and has a genome size of ~ 45 Kb. Molecular data based on the gene which encodes for
the phage tail tubular protein suggests that the phage is distinct from known phages but related to phages which
infect B. thailandensis and Ralstonia spp. The phage FBp-AMP1 is the first B. pseudomallei podovirus to be isolated
from the environment rather than being induced from a bacterial culture. It has a broad host range within B.
pseudomallei and can infect all 11 strains that we tested it on but not related Burkholderia species. It is heat stable
for 8 h at 50°C but not stable at 60°C. It may potentially be a useful tool to treat or diagnose B. pseudomallei
infections as it can lyse several strains of clinical relevance.