Cytochalasin E: Inhibition of intestinal glucose absorption in the mouse

Abstract

In situ glucose absorption in the mouse was significantly inhibited by cytochalasin E. Cytochalasin E (5 mg/ml) also inhibited glucose absorption up to 55.5% in mouse jejunum in vitro. During its inhibition transmural potential difference (PD) was increased from -7.4 to -0.4 mV, together with a decrease in glucose accumulation in the intestinal tissues. Furthermore, it was also found that cytochalasin E induced an alteration in Kmvalue from 2.9 · 10-3to 4.0 · 10-2M and a constant Vmaxvalue of 55.5 smmol/100 mg wet wt tissue/min. It is postulated that cytochalasin E is a possible competitive inhibitor of glucose at the receptor sites of carriers on the microvillar membrane of the intestinal absorptive cells. © 1983.