Publication: Effects of thymidine phosphorylase on tumor aggressiveness and 5-fluorouracil sensitivity in cholangiocarcinoma
Issued Date
2010
Resource Type
Language
eng
ISSN
1007-9327
1007-9327 (print)
1007-9327 (print)
Bibliographic Citation
World J Gastroenterol. Vol.16, No.13 (2010), 1631-1638
Suggested Citation
Jongkonnee Thanasai, Temduang Limpaiboon, Patcharee Jearanaikoon, Banchob Sripa, Chawalit Pairojkul, Srisurang Tantimavanich,, Masanao Miwa Effects of thymidine phosphorylase on tumor aggressiveness and 5-fluorouracil sensitivity in cholangiocarcinoma. World J Gastroenterol. Vol.16, No.13 (2010), 1631-1638. doi:doi:10.3748/wjg.v16.i13.1631 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2073
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Title
Effects of thymidine phosphorylase on tumor aggressiveness and 5-fluorouracil sensitivity in cholangiocarcinoma
Abstract
AIM: To evaluate the role of thymidine phosphorylase
(TP) in cholangiocarcinoma using small interfering RNA
(siRNA).
METHODS: A human cholangiocarcinoma-derived cell
line KKU-M139, which has a naturally high level of endogenous
TP, had TP expression transiently knocked down
using siRNA. Cell growth, migration, in vitro angiogenesis,
apoptosis, and cytotoxicity were assayed in TP
knockdown and wild-type cell lines.
RESULTS: TP mRNA and protein expression were
decreased by 87.1% ± 0.49% and 72.5% ± 3.2%,
respectively, compared with control cells. Inhibition of
TP significantly decreased migration of KKU-M139, and
suppressed migration and tube formation of human
umbilical vein endothelial cells. siRNA also reduced the
ability of TP to resist hypoxia-induced apoptosis, while
suppression of TP reduced the sensitivity of KKU-M139
to 5-fluorouracil.
CONCLUSION: Inhibition of TP may be beneficial in
decreasing angiogenesis-dependent growth and migration
of cholangiocarcinoma but may diminish the response
to 5-fluorouracil chemotherapy.
