Publication: Heavy metal resistant anaerobic bacterial strains from brewery digester sludge
Issued Date
2013-01-01
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ISSN
17582091
17582083
17582083
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2-s2.0-84877753922
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Global Warming. Vol.5, No.2 (2013), 127-134
Suggested Citation
Nusara Sinbuathong, Pramote Sirirote, Daniel Watts, Suphang Chulalaksananukul Heavy metal resistant anaerobic bacterial strains from brewery digester sludge. International Journal of Global Warming. Vol.5, No.2 (2013), 127-134. doi:10.1504/IJGW.2013.053494 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31695
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Title
Heavy metal resistant anaerobic bacterial strains from brewery digester sludge
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Abstract
This work focused on the study of the types of heavy-metal-resistant anaerobic bacteria from the sludge of a brewery wastewater treatment plant exposed to high concentrations of dissolved Cd (II), Cu (II) and Zn (II). Characterisations were carried out by polymerase chain reaction of the 16S rRNA gene of bacterial strain. Using special culture media, two types of strong heavy metal-resistant bacterial strains were isolated. The first was a sulphate-reducing bacterium identified as Clostridium ganghwense strain HY-42-06. This strain of sulphate-reducing bacteria tolerated Cd (II), Cu (II) and Zn (II) at the tested concentrations. The other type was identified as consisting of a mixture of Micrococcus luteus, Wolinella succinogenes, Sporosarscina sp. PIC-C28 and Alicycliphilus sp. R-24604. The results found that these four dominant strains tolerated Cd (II) at 20 mg/l, only Wolinella succinogenes cannot tolerate Cu (II) at 2 mg/l while none of them tolerated Zn (II) at 30 mg/l. Copyright © 2013 Inderscience Enterprises Ltd.