Publication: An integration-free iPSC line (MUSIi008-A) derived from a patient with severe hemolytic anemia carrying compound heterozygote mutations in KLF1 gene for disease modeling
| dc.contributor.author | Ponthip Potirat | en_US |
| dc.contributor.author | Methichit Wattanapanitch | en_US |
| dc.contributor.author | Vip Viprakasit | en_US |
| dc.contributor.author | Pakpoom Kheolamai | en_US |
| dc.contributor.author | Surapol Issaragrisil | en_US |
| dc.contributor.other | Faculty of Medicine, Thammasat University | en_US |
| dc.contributor.other | Faculty of Medicine, Siriraj Hospital, Mahidol University | en_US |
| dc.date.accessioned | 2020-01-27T07:55:50Z | |
| dc.date.available | 2020-01-27T07:55:50Z | |
| dc.date.issued | 2019-01-01 | en_US |
| dc.description.abstract | © 2018 The Authors We generated an induced pluripotent stem cell (iPSC) line from peripheral blood mononuclear cells (PBMNCs) isolated from a 1-year old female carrying compound heterozygote for KLF1 mutations (G176RfsX179 and A298P mutations). Non-integrating Sendai viral (SeV) vectors containing KOS, hc-MYC and hKLF4 were used for reprogramming. The established MUSIi008-A cell line contained the same mutations found in the patient, expressed pluripotent markers, differentiated into cells of three embryonic germ layers both in vitro and in vivo, and exhibited normal karyotype. This cell line may provide an alternative renewable source of cells for in vitro disease modeling of severe transfusion-dependent hemolytic anemia. | en_US |
| dc.identifier.citation | Stem Cell Research. Vol.34, (2019) | en_US |
| dc.identifier.doi | 10.1016/j.scr.2018.09.021 | en_US |
| dc.identifier.issn | 18767753 | en_US |
| dc.identifier.issn | 18735061 | en_US |
| dc.identifier.other | 2-s2.0-85059233299 | en_US |
| dc.identifier.uri | https://repository.li.mahidol.ac.th/handle/123456789/50364 | |
| dc.rights | Mahidol University | en_US |
| dc.rights.holder | SCOPUS | en_US |
| dc.source.uri | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85059233299&origin=inward | en_US |
| dc.subject | Biochemistry, Genetics and Molecular Biology | en_US |
| dc.title | An integration-free iPSC line (MUSIi008-A) derived from a patient with severe hemolytic anemia carrying compound heterozygote mutations in KLF1 gene for disease modeling | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| mu.datasource.scopus | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85059233299&origin=inward | en_US |