Publication: Polymerase chain reaction detection of Plasmodium falciparum in mosquitoes
2
Issued Date
1993-01-01
Resource Type
ISSN
18783503
00359203
00359203
Other identifier(s)
2-s2.0-0027268678
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Mahidol University
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SCOPUS
Bibliographic Citation
Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.87, No.3 (1993), 273-275
Suggested Citation
V. Boonsaeng, S. Panyim, P. Wilairat, A. Tassanakajon Polymerase chain reaction detection of Plasmodium falciparum in mosquitoes. Transactions of the Royal Society of Tropical Medicine and Hygiene. Vol.87, No.3 (1993), 273-275. doi:10.1016/0035-9203(93)90124-9 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/22586
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Title
Polymerase chain reaction detection of Plasmodium falciparum in mosquitoes
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Abstract
The polymerase chain reaction (PCR) procedure using a primer set derived from a repetitive deoxyribonucleic acid (DNA) sequence specific to Plasmodium falciparum was used to detect parasite DNA in mosquitoes. In laboratory-infected mosquitoes, PCR could detect as few as 10 sporozoites in a dissected salivary gland and a single oocyst in a dissected midgut. The ability to detect P. falciparum DNA in wild-caught mosquitoes indicated an advantage of the PCR over enzyme-linked immunosorbent assay for the detection of Plasmodium sporozoites in mosquitoes with low-grade parasite infections. © 1993 Oxford University Press.