Publication:
Attenuation of oxidant-induced muscle insulin resistance and p38 MAPK by exercise training

dc.contributor.authorKanokwan Vichaiwongen_US
dc.contributor.authorErik J. Henriksenen_US
dc.contributor.authorChaivat Toskulkaoen_US
dc.contributor.authorMujalin Prasannarongen_US
dc.contributor.authorTepmanas Bupha-Intren_US
dc.contributor.authorVitoon Saengsirisuwanen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherUniversity of Arizona College of Medicine - Tucsonen_US
dc.contributor.otherThailand Ministry of Science and Technologyen_US
dc.date.accessioned2018-09-13T06:22:17Z
dc.date.available2018-09-13T06:22:17Z
dc.date.issued2009-09-01en_US
dc.description.abstractWe have recently shown that direct exposure to an oxidant stress induces resistance to insulin in glucose transport activity in intact rat skeletal muscle. In this study, we evaluated the effectiveness of prior exercise training in attenuating oxidative stress-induced insulin resistance. Male Sprague-Dawley rats either remained sedentary or underwent a treadmill-running regimen for 6 weeks. Isolated soleus muscles were incubated in the absence or presence of hydrogen peroxide (H2O2) (50-70 μM) with or without insulin for 2 h. In the sedentary animals, H2O2significantly inhibited insulin action on glucose transport activity and phosphorylation of Akt (Ser473), by 28 and 24%, respectively, and substantially activated the phosphorylation levels of p38 MAPK (Thr180/Tyr182) by 43% and SAPK/JNK (Thr183/Tyr185) by 111%. Interestingly, the inhibitory effects of H2O2on insulin-stimulated glucose transport and Akt (Ser473) phosphorylation were attenuated by 43 and 75% in exercise-trained muscles. Additionally, the phosphorylation level of p38 MAPK (Thr180/Tyr182) triggered by oxidative stress was reduced by 59% in the exercise-trained muscle. We have demonstrated for the first time in mammalian skeletal muscle that endurance exercise training can partially protect against glucose transport resistance to insulin induced by oxidative stress, and this benefit of exercise training is at least in part mediated through the insulin signaling pathway and stress-activated signaling elements. © 2009 Elsevier Inc.en_US
dc.identifier.citationFree Radical Biology and Medicine. Vol.47, No.5 (2009), 593-599en_US
dc.identifier.doi10.1016/j.freeradbiomed.2009.05.036en_US
dc.identifier.issn08915849en_US
dc.identifier.other2-s2.0-67650725419en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/123456789/27153
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67650725419&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectMedicineen_US
dc.titleAttenuation of oxidant-induced muscle insulin resistance and p38 MAPK by exercise trainingen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67650725419&origin=inwarden_US

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