Publication: Molecular evidence of speciation between island and continental populations of Anopheles (Cellia) sundaicus (Diptera: Culicidae), a principal malaria vector taxon in Southeast Asia
Issued Date
2004-01-01
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ISSN
00222585
Other identifier(s)
2-s2.0-2542554354
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Medical Entomology. Vol.41, No.3 (2004), 287-295
Suggested Citation
Isabelle Dusfour, Yvonne Marie Linton, Anna Cohuet, Ralph E. Harbach, Visut Baimai, H. O.D. Trung, Chang Moh Seng, Asmad Matusop, Sylvie Manguin Molecular evidence of speciation between island and continental populations of Anopheles (Cellia) sundaicus (Diptera: Culicidae), a principal malaria vector taxon in Southeast Asia. Journal of Medical Entomology. Vol.41, No.3 (2004), 287-295. doi:10.1603/0022-2585-41.3.287 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/21106
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Title
Molecular evidence of speciation between island and continental populations of Anopheles (Cellia) sundaicus (Diptera: Culicidae), a principal malaria vector taxon in Southeast Asia
Abstract
Anopheles sundaicus s.l. is a principal malaria vector taxon on islands and along the coastal areas of Southeast Asia. It has a wide geographical distribution and exhibits a high level of ecological and behavioral variability. Study of this taxon is crucial for understanding its biology and implementing effective vector control measures. We compared populations of An. sundaicus from Vietnam, Thailand, and Malaysian Borneo by using two mitochondrial DNA markers: cytochrome oxidase I and cytochrome b. Genetic divergence, geographic separation, and cladistic analysis of relationships revealed the presence of two cryptic species: Anopheles sundaicus s.s. on Malaysian Borneo and An. sundaicus species A in coastal areas of Thailand and Vietnam. A polymerase chain reaction (PCR) assay was developed to easily identify these two species throughout their geographic distributions. The assay was based on sequence characterized amplified region derived from random amplified polymorphic DNA. This PCR identification method needs to be validated and adapted for the recognition of other possible species in the Sundaicus Complex.