Publication: A Case-Control Study of Involvement of Oxidative DNA Damage and Alteration of Antioxidant Defense System in Patients with Basal Cell Carcinoma: Modulation by Tumor Removal
Issued Date
2016-01-01
Resource Type
ISSN
19420994
19420900
19420900
Other identifier(s)
2-s2.0-84962816204
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Oxidative Medicine and Cellular Longevity. Vol.2016, (2016)
Suggested Citation
Lapatsanant Chaisiriwong, Rungsima Wanitphakdeedecha, Panitta Sitthinamsuwan, Somponnat Sampattavanich, Somruedee Chatsiricharoenkul, Woraphong Manuskiatti, Uraiwan Panich A Case-Control Study of Involvement of Oxidative DNA Damage and Alteration of Antioxidant Defense System in Patients with Basal Cell Carcinoma: Modulation by Tumor Removal. Oxidative Medicine and Cellular Longevity. Vol.2016, (2016). doi:10.1155/2016/5934024 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/43170
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
A Case-Control Study of Involvement of Oxidative DNA Damage and Alteration of Antioxidant Defense System in Patients with Basal Cell Carcinoma: Modulation by Tumor Removal
Other Contributor(s)
Abstract
© 2016 Lapatsanant Chaisiriwong et al. Oxidative damage has been suggested to play a role in the pathogenesis of basal cell carcinoma (BCC). This study illustrated an involvement of oxidative DNA damage and changes in antioxidant defenses in BCC by conducting a case-control study (24 controls and 24 BCC patients) and assessing urinary 7,8-dihydro-8-oxo-2′-deoxyguanosine (8-oxo-dGuo), plasma antioxidant defenses including catalase (CAT), glutathione peroxidase (GPx), NQO1, and total superoxide dismutase (SOD) activities, and glutathione (GSH) levels before surgery and 1 month after surgery. 8-oxo-dGuo expressions as well as protein and mRNA expressions of DNA repair enzyme hOGG1 and antioxidant defenses (CAT, GCLC, GPx, Nrf2, and MnSOD) in nonneoplastic epidermis of control and BCC tissues were also determined. This study observed induction in urinary 8-oxo-dGuo, increased 8-oxo-dGuo expression, and reduced hOGG1 protein and mRNA in BCC tissues, decreased activities of CAT, GPx, and NQO1, but elevated SOD activities and GSH levels in BCC patients and reduction of all antioxidant proteins and genes studied in BCC tissues. Furthermore, decreased plasma antioxidant activities in BCC patients were restored at 1 month after operation compared with preoperative levels. Herein, we concluded that BCC patients were associated with oxidative DNA damage and depletion of antioxidant defenses and surgical removal of BCC correlated with improved redox status.