Asian Pacific Journal of Allergy and Immunology. Vol.5, No.2 (1987), 143-148
Suggested Citation
C. Yoosook, P. Rimdusit, W. Chantratita, P. Leechanachai, P. Bhattarakosol Evaluation of biotin-streptavidin enzyme-linked immunosorbent assay for detection of genital herpes simplex virus infection.. Asian Pacific Journal of Allergy and Immunology. Vol.5, No.2 (1987), 143-148. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/15341
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Evaluation of biotin-streptavidin enzyme-linked immunosorbent assay for detection of genital herpes simplex virus infection.
A biotin-streptavidin enzyme-linked immunosorbent assay (B-SA ELISA) was evaluated for detection of herpes simplex virus (HSV) in clinical specimens which were cervico-vaginal swabs from 205 asymptomatic women and swabs from the genital lesions of 163 suspected patients. All specimens were also subjected to a conventional virus isolation in cell culture. A blocking B-SA ELISA had 100% specificity and 98% sensitivity compared with viral isolation from patients, but had only 40% sensitivity using specimens from asymptomatics. The conventional B-SA ELISA might also be used; it gave results corresponding to B-SA ELISA blocking test except for a single specimen which was considered a false positive.
