Publication: Graphene-based electrochemical genosensor incorporated loop-mediated isothermal amplification for rapid on-site detection of Mycobacterium tuberculosis
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Issued Date
2020-07-15
Resource Type
ISSN
1873264X
07317085
07317085
Other identifier(s)
2-s2.0-85084296211
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Pharmaceutical and Biomedical Analysis. Vol.186, (2020)
Suggested Citation
Wansadaj Jaroenram, Jantana Kampeera, Narong Arunrut, Chanpen Karuwan, Assawapong Sappat, Pakapreud Khumwan, Sarinya Jaitrong, Kobporn Boonnak, Therdsak Prammananan, Angkana Chaiprasert, Adisorn Tuantranont, Wansika Kiatpathomchai Graphene-based electrochemical genosensor incorporated loop-mediated isothermal amplification for rapid on-site detection of Mycobacterium tuberculosis. Journal of Pharmaceutical and Biomedical Analysis. Vol.186, (2020). doi:10.1016/j.jpba.2020.113333 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/56107
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Title
Graphene-based electrochemical genosensor incorporated loop-mediated isothermal amplification for rapid on-site detection of Mycobacterium tuberculosis
Abstract
© 2020 Elsevier B.V. Tuberculosis (TB) is one of the most contagious and lethal infectious diseases that affects more than 10 million individuals worldwide. A lack of rapid TB diagnosis is partly responsible for its alarming spread and prevalence in many regions. To address this problem, we report a novel integrated point-of-care platform to detect a TB-causative bacterium, Mycobacterium tuberculosis (Mtb). This leverages loop-mediated isothermal amplification (LAMP) for Mtb-DNA amplification and the screen-printed graphene electrode (SPGE) for label-free electrochemical analysis of DNA amplicons. When implemented on a portable potentiostat device developed in-house, the system (LAMP-EC) offers a rapid end-point qualitative analysis of specific DNA amplicons that will be displayed as a discrete positive/negative readout on the LCD screen. Under optimized conditions, LAMP-EC showed a comparable detection limit to the previously developed LAMP assay with a lateral flow readout at 1 pg total DNA or 40 Mtb genome equivalents. This highly specific technique detected the presence of TB in all 104 blinded sputum samples with a 100% accuracy. Our technique can also easily be clinically adopted due to its affordability (∼USD2.5/test), rapidity (<65 min turnaround time) and feasibility (lack of advanced instrumental requirement). This serves as a practical incentive, appealing to users in both high- and low-resource settings across the TB endemic regions and economic backgrounds.