Publication: Gene expression profiling of intrahepatic cholangiocarcinoma
Issued Date
2013-01-01
Resource Type
ISSN
2476762X
15137368
15137368
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2-s2.0-84877145474
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Pacific Journal of Cancer Prevention. Vol.14, No.1 (2013), 557-563
Suggested Citation
Ittisak Subrungruang, Charin Thawornkuno, Chawalitchewinkoon Petmitr Porntip, Chawalit Pairojkul, Sopit Wongkham, Songsak Petmitr Gene expression profiling of intrahepatic cholangiocarcinoma. Asian Pacific Journal of Cancer Prevention. Vol.14, No.1 (2013), 557-563. doi:10.7314/APJCP.2013.14.1.557 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/31392
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Title
Gene expression profiling of intrahepatic cholangiocarcinoma
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Abstract
Intrahepatic cholangiocarcinoma (ICC) is ranked as one of the top five causes of cancer-related deaths. ICC in Thai patients is associated with infection with the liver fluke, Opisthorchis viverrini, but the molecular basis for development remains unclear. The present study employed a microarray approach to compare gene expression profiles of ICCs and normal liver tissues from the same patients residing in Northeast Thailand, a region with a high prevalence of liver fluke infection. In ICC samples, 2,821 and 1,361 genes were found to be significantly up- and down-regulated respectively (unpaired t-test, p<0.05; fold-change =2.0). For validation of the microarray results, 7 up-regulated genes (FXYD3, GPRC5A, CEACAM5, MUC13, EPCAM, TMC5, and EHF) and 3 down-regulated genes (CPS1, TAT, and ITIH1) were selected for confirmation using quantitative RT-PCR, resulting in 100% agreement. The metallothionine heavy metal pathway contains the highest percentage of genes with statistically significant changes in expression. This study provides exon-level expression profiles in ICC that should be fruitful in identifying novel genetic markers for classifying and possibly early diagnosis of this highly fatal type of cholangiocarcinoma.