Publication: Development of DNA controls for detection of β-thalassemia mutations commonly found in Asian
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Issued Date
2020-01-01
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ISSN
1751553X
17515521
17515521
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2-s2.0-85088398905
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal of Laboratory Hematology. (2020)
Suggested Citation
Thongperm Munkongdee, Tiwaporn Nualkaew, Nattrika Buasuwan, Nurmeeha Hinna, Kittiphong Paiboonsukwong, Orapan Sripichai, Saovaros Svasti, Pranee Winichagoon, Suthat Fucharoen, Natee Jearawiriyapaisarn Development of DNA controls for detection of β-thalassemia mutations commonly found in Asian. International Journal of Laboratory Hematology. (2020). doi:10.1111/ijlh.13292 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/57759
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Title
Development of DNA controls for detection of β-thalassemia mutations commonly found in Asian
Abstract
© 2020 John Wiley & Sons Ltd Introduction: Several DNA-based approaches including a reverse dot-blot hybridization (RDB) have been established for detection of β-thalassemia genotypes to provide accurate genetic counseling and prenatal diagnosis for prevention and control of severe β-thalassemia. However, one of major concerns of these techniques is a risk of misdiagnosis due to a lack of DNA controls. Here, we constructed positive DNA controls for β-thalassemia genotyping in order to ensure that all steps in the analysis are performed properly. Methods: Four recombinant β-globin plasmids, including a normal sequence and three different mutant panels covering 10 common β-thalassemia mutations in Asia, were constructed by a conventional cloning method followed by sequential rounds of site-directed mutagenesis. These positive DNA controls were further validated by RDB analysis. Results: We demonstrated the applicability of established positive DNA controls for β-thalassemia genotyping in terms of accuracy and reproducibility by RDB analysis. We further combined three mutant β-globin plasmids into a single positive control, which showed positive signals for both normal and mutant probes of all tested mutations. Therefore, only two positive DNA controls, normal and combined mutant β-globin plasmids, are required for detecting 10 common β-thalassemia mutations by RDB, reducing the cost, time, and efforts in the routine diagnosis. Conclusion: The β-globin DNA controls established here provide efficient alternatives to a conventional DNA source from peripheral blood, which is more difficult to obtain. They also provide a platform for future development of β-globin plasmid controls with other mutations, which can also be suitable for other DNA-based approaches.