Publication: Inhibitory effects of erythrosine/curcumin derivatives/nano-titanium dioxide-mediated photodynamic therapy on Candida albicans
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Issued Date
2021-04-21
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ISSN
14203049
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2-s2.0-85105159288
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecules. Vol.26, No.9 (2021)
Suggested Citation
Kasama Kanpittaya, Aroon Teerakapong, Noppawan Phumala Morales, Doosadee Hormdee, Aroonsri Priprem, Wilawan Weera-Archakul, Teerasak Damrongrungruang Inhibitory effects of erythrosine/curcumin derivatives/nano-titanium dioxide-mediated photodynamic therapy on Candida albicans. Molecules. Vol.26, No.9 (2021). doi:10.3390/molecules26092405 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76206
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Title
Inhibitory effects of erythrosine/curcumin derivatives/nano-titanium dioxide-mediated photodynamic therapy on Candida albicans
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Abstract
This study focuses on the role of photosensitizers in photodynamic therapy. The photosen-sitizers were prepared in combinations of 110/220 µM erythrosine and/or 10/20 µM demethoxy/ bisdemethoxy curcumin with/without 10% (w/w) nano-titanium dioxide. Irradiation was performed with a dental blue light in the 395–480 nm wavelength range, with a power density of 3200 mW/cm2 and yield of 72 J/cm2. The production of ROS and hydroxyl radical was investigated using an electron paramagnetic resonance spectrometer for each individual photosensitizer or in photosen-sitizer combinations. Subsequently, a PrestoBlue® toxicity test of the gingival fibroblast cells was performed at 6 and 24 h on the eight highest ROS-generating photosensitizers containing curcumin derivatives and erythrosine 220 µM. Finally, the antifungal ability of 22 test photosensitizers, Candida albicans (ATCC 10231), were cultured in biofilm form at 37◦C for 48 h, then the colonies were counted in colony-forming units (CFU/mL) via the drop plate technique, and then the log reduction was calculated. The results showed that at 48 h the test photosensitizers could simultaneously produce both ROS types. All test photosensitizers demonstrated no toxicity on the fibroblast cells. In total, 18 test photosensitizers were able to inhibit Candida albicans similarly to nystatin. Conclusively, 20 µM bisdemethoxy curcumin + 220 µM erythrosine + 10% (w/w) nano-titanium dioxide exerted the highest inhibitory effect on Candida albicans.