Publication: Identification of genes involved in the butyrolactone autoregulator cascade that modulates secondary metabolism in Streptomyces lavendulae FRI-5
Issued Date
2008-12-01
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ISSN
03781119
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2-s2.0-53149128699
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Mahidol University
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SCOPUS
Bibliographic Citation
Gene. Vol.425, No.1-2 (2008), 9-16
Suggested Citation
Shigeru Kitani, Aya Iida, Taka aki Izumi, Asa Maeda, Yasuhiro Yamada, Takuya Nihira Identification of genes involved in the butyrolactone autoregulator cascade that modulates secondary metabolism in Streptomyces lavendulae FRI-5. Gene. Vol.425, No.1-2 (2008), 9-16. doi:10.1016/j.gene.2008.07.043 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18812
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Title
Identification of genes involved in the butyrolactone autoregulator cascade that modulates secondary metabolism in Streptomyces lavendulae FRI-5
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Abstract
The γ-butyrolactone-autoregulator signalling system is widely distributed across many Streptomyces species and it controls secondary metabolism and/or morphological differentiation. IM-2 [(2R,3R,1′R)-2-1′-hydroxybutyl-3-hydroxymethyl-γ-butanolide] is a γ-butyrolactone autoregulator which, in Streptomyces lavendulae FRI-5, switches off the production of d-cycloserine, but switches on the production of several nucleoside antibiotics and blue pigment. In the IM-2 system, an IM-2 specific receptor (FarA) plays a critical role in the biosynthetic regulation of these metabolites, including IM-2 itself. Here, we identified five additional regulatory genes in the farA-flanking region and demonstrated that, in addition to farA, at least two more genes (farR1 and farR2) are involved in the IM-2/FarA system as the direct transcriptional target of FarA. The gel-shift assay revealed that FarA was bound to the upstream region of the four genes (including farR1 and farR2) in an IM-2-dependent manner. The FarA-binding sites were localized by DNase I footprinting to 27- to 33-bp palindromic structures, suggesting that FarA-binding sequences consist of two conserved hexamers separated by six nucleotides. Both farR1 and farR2 were transcribed in a growth-dependent manner, and marked expression was induced in the presence of IM-2, whereas transcripts of other two genes were not detected under the cultivation conditions used. The FarA-binding sites of farR1 and far2 overlap the promoter regions, suggesting that FarA represses the transcription of these two genes in the absence of IM-2 by inhibiting RNA polymerase access. © 2008 Elsevier B.V. All rights reserved.