Publication: Effects of thymidine phosphorylase on tumor aggressiveness and 5-fluorouracil sensitivity in cholangiocarcinoma
Issued Date
2010-04-07
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ISSN
10079327
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2-s2.0-77950901564
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Mahidol University
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SCOPUS
Bibliographic Citation
World Journal of Gastroenterology. Vol.16, No.13 (2010), 1631-1638
Suggested Citation
Jongkonnee Thanasai, Temduang Limpaiboon, Patcharee Jearanaikoon, Banchob Sripa, Chawalit Pairojkul, Srisurang Tantimavanich, Masanao Miwa Effects of thymidine phosphorylase on tumor aggressiveness and 5-fluorouracil sensitivity in cholangiocarcinoma. World Journal of Gastroenterology. Vol.16, No.13 (2010), 1631-1638. doi:10.3748/wjg.v16.i13.1631 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/29706
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Title
Effects of thymidine phosphorylase on tumor aggressiveness and 5-fluorouracil sensitivity in cholangiocarcinoma
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Abstract
Aim: To evaluate the role of thymidine phosphorylase (TP) in cholangiocarcinoma using small interfering RNA (siRNA). Methods: A human cholangiocarcinoma-derived cell line KKU-M139, which has a naturally high level of endogenous TP, had TP expression transiently knocked down using siRNA. Cell growth, migration, in vitro angiogenesis, apoptosis, and cytotoxicity were assayed in TP knockdown and wild-type cell lines. Results: TP mRNA and protein expression were decreased by 87.1% ± 0.49% and 72.5% ± 3.2%, respectively, compared with control cells. Inhibition of TP significantly decreased migration of KKU-M139, and suppressed migration and tube formation of human umbilical vein endothelial cells. siRNA also reduced the ability of TP to resist hypoxia-induced apoptosis, while suppression of TP reduced the sensitivity of KKU-M139 to 5-fluorouracil. Conclusion: Inhibition of TP may be beneficial in decreasing angiogenesis-dependent growth and migration of cholangiocarcinoma but may diminish the response to 5-fluorouracil chemotherapy. © 2010 Baishideng.