Publication: Gonadotropin-releasing hormone (gnrh) and a gnrh analog induce ovarian maturation in the giant freshwater prawn, macrobrachium rosenbergii
Issued Date
2009-01-01
Resource Type
ISSN
07924259
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2-s2.0-73449110931
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Mahidol University
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SCOPUS
Bibliographic Citation
Invertebrate Reproduction and Development. Vol.53, No.3 (2009), 125-135
Suggested Citation
Piyada Ngernsoungnern, Apichart Ngernsoungnern, Prasert Sobhon, Prapee Sretarugsa Gonadotropin-releasing hormone (gnrh) and a gnrh analog induce ovarian maturation in the giant freshwater prawn, macrobrachium rosenbergii. Invertebrate Reproduction and Development. Vol.53, No.3 (2009), 125-135. doi:10.1080/07924259.2009.9652298 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/27069
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Title
Gonadotropin-releasing hormone (gnrh) and a gnrh analog induce ovarian maturation in the giant freshwater prawn, macrobrachium rosenbergii
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Abstract
Gonadotropin-releasing hormone (GnRH) is a highly conserved peptide that plays a role in regulating reproduction in both vertebrates and invertebrates. The present study investigated the effects of lamprey (l)GnRH-I, lGnRH-III, octopus (oct)GnRH and buserelin, a GnRH analog (GnRHa), on ovarian maturation and spawning in the giant freshwater prawn, Macrobrachium rosenbergii. We demonstrated that the times for ovarian maturation in prawns treated with octGnRH (at 50 and 500 ng/g BW), lGnRH-I and lGnRH-III (at 500 ng/g BW), and GnRHa (at 1,000 ng/g BW) were significantly shorter (23.50 ± 2.12 and 22.50 ± 1.15, 25.50 ± 4.04 and 25.50± 4.03, and 26.67 ± 4.04 days) than the controls (40.0 ± 3.40 days). On day 22 post-treatment, the gonadosomatic index (GSI) values of the prawns treated with octGnRH (at 50 and 500 ng/g BW), lGnRH-I and lGnRH-III (at 500 ng/g BW), and GnRHa (at 1,000 ng/g BW) were significantly greater (2.50 ± 0.72 and 4.64 ± 0.95, 6.07 ± 1.29 and 8.90 ± 1.04, and 3.88 ± 1.34%) than the controls (0.45 ± 0.15%). Vitellin protein was first detected in the ovary on day 15 and had increased significantly by day 22 in prawns treated with GnRHs at 500 ng/g BW, while it was not detected in the controls. The prawns treated with the GnRHs and GnRHa showed similar numbers and percentages of spawned and fertilized eggs to those of the control group. These findings indicate that GnRH controls ovarian maturation and spawning in this prawn, as in other species. © 2009 Taylor & Francis Group, LLC.