Publication: DNA barcode identification of freshwater snails in the family bithyniidae from Thailand
Issued Date
2013-11-04
Resource Type
ISSN
19326203
Other identifier(s)
2-s2.0-84892580969
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS ONE. Vol.8, No.11 (2013)
Suggested Citation
Jutharat Kulsantiwong, Sattrachai Prasopdee, Jiraporn Ruangsittichai, Wipaporn Ruangjirachuporn, Thidarut Boonmars, Vithoon Viyanant, Paola Pierossi, Paul D N Hebert, Smarn Tesana DNA barcode identification of freshwater snails in the family bithyniidae from Thailand. PLoS ONE. Vol.8, No.11 (2013). doi:10.1371/journal.pone.0079144 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/30955
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
DNA barcode identification of freshwater snails in the family bithyniidae from Thailand
Other Contributor(s)
Abstract
Freshwater snails in the family Bithyniidae are the first intermediate host for Southeast Asian liver fluke (Opisthorchis viverrini), the causative agent of opisthorchiasis. Unfortunately, the subtle morphological characters that differentiate species in this group are not easily discerned by non-specialists. This is a serious matter because the identification of bithyniid species is a fundamental prerequisite for better understanding of the epidemiology of this disease. Because DNA barcoding, the analysis of sequence diversity in the 5' region of the mitochondrial COI gene, has shown strong performance in other taxonomic groups, we decided to test its capacity to resolve 10 species/ subspecies of bithyniids from Thailand. Our analysis of 217 specimens indicated that COI sequences delivered species-level identification for 9 of 10 currently recognized species. The mean intraspecific divergence of COI was 2.3% (range 0-9.2%), whereas sequence divergences between congeneric species averaged 8.7% (range 0-22.2%). Although our results indicate that DNA barcoding can differentiate species of these medically-important snails, we also detected evidence for the presence of one overlooked species and one possible case of synonymy. © 2013 Kulsantiwong et al.